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Cellosaurus HET-1A (CVCL_3702)

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Cell line name HET-1A
Synonyms Het-1A; HET1A; Het1A
Accession CVCL_3702
Resource Identification Initiative To cite this cell line use: HET-1A (RRID:CVCL_3702)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10209.
Population: African American.
Doubling time: 27.4 +- 3.6 hours (PubMed=21127259).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRSV-T).
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Caution: Indicated as originating from a 25 year old male in ATCC, but said to be derived from autopsy specimen from a 74 year old male.
Derived from site: In situ; Esophagus; UBERON=UBERON_0001043.
Cell type: Epithelial cell of esophagus; CL=CL_0002252.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_4T84 (HET1AR)
Sex of cell Male
Age at sampling 74Y
Category Transformed cell line
STR profile Source(s): ATCC=CRL-2692

Markers:
AmelogeninX,Y
CSF1PO10,12
D2S133817,23
D3S135815,17
D5S81811,12
D7S8209
D8S117913,14
D13S31711
D16S5399,11
D18S5114
D19S43312,14
D21S1128,31
FGA22,25
Penta D12,13
Penta E7
TH017
TPOX11
vWA16

Run an STR similarity search on this cell line
Publications

CLPUB00265
Stoner G.D., Kaighn M.E., Resau J.H., Naito Z., Bowman D., Harris C.C.
Immortalized human esophageal epithelial cell lines for studies of carcinogen-induced cell transformation.
In Vitro Cell. Dev. Biol. 26:23A-23A(1990)

DOI=10.1007/978-1-4612-0411-4_30
Stoner G.D., Light B.A., Harris C.C.
Immortalization and oncogene transformation of human esophageal epithelial cells.
(In book chapter) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim J.S., Dritschilo A. (eds.); pp.297-305; Humana Press; New York; USA (1990)

Patent=WO1991005062A1
Stoner G.D., Reddel R.R., Harris C.C.
Human esophageal epithelial cell lines.
Patent number WO1991005062A1, 18-Apr-1991

PubMed=1703038
Stoner G.D., Kaighn M.E., Reddel R.R., Resau J.H., Bowman D., Naito Z., Matsukura N., You M., Galati A.J., Harris C.C.
Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells.
Cancer Res. 51:365-371(1991)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Lehman T.A., Modali R.V., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

PubMed=7972006; DOI=10.1073/pnas.91.23.11045; PMCID=PMC45163
Okamoto A., Demetrick D.J., Spillare E.A., Hagiwara K., Hussain S.P., Bennett W.P., Forrester K., Gerwin B.I., Serrano M., Beach D.H., Harris C.C.
Mutations and altered expression of p16INK4 in human cancer.
Proc. Natl. Acad. Sci. U.S.A. 91:11045-11049(1994)

PubMed=10078940; DOI=10.1002/(SICI)1098-2744(199902)24:2<128::AID-MC7>3.0.CO;2-F
Compton K.R., Orringer M.B., Beer D.G.
Induction of glutathione s-transferase-pi in Barrett's metaplasia and Barrett's adenocarcinoma cell lines.
Mol. Carcinog. 24:128-136(1999)

PubMed=20139130; DOI=10.1093/carcin/bgq036
Duggan S.P., Behan F.M., Kirca M., Smith S.M., Reynolds J.V., Long A., Kelleher D.P.
An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels.
Carcinogenesis 31:936-945(2010)

PubMed=20843300; DOI=10.1042/BC20100071
Underwood T.J., Derouet M.F., White M.J., Noble F., Moutasim K.A., Smith E., Drew P.A., Thomas G.J., Primrose J.N., Blaydes J.P.
A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture.
Biol. Cell 102:635-644(2010)

PubMed=21127259; DOI=10.1152/ajpgi.00461.2010; PMCID=PMC3043651
Goldman A., Chen H.D.R., Roesly H.B., Hill K.A., Tome M.E., Dvorak B., Bernstein H., Dvorak K.
Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis.
Am. J. Physiol. Gastrointest. Liver Physiol. 300:G292-G302(2011)

DOI=10.1155/2014/376541
Akagi I., Ishibashi O., Matsutani T., Hagiwara N., Matsuda A., Nomura T., Makino H., Yoshida H., Miyashita M., Uchida E.
Comprehensive analysis of microRNA and mRNA expression in normal and tumorous human esophageal squamous cell lines using microarray datasets.
Dataset Papers Sci. 2013:376541.1-376541.3(2013)

PubMed=28224081; DOI=10.1016/j.gdata.2017.02.002; PMCID=PMC5304241
Akagi I., Ishibashi O.
RNA-Seq analysis of human cell lines established from normal and neoplastic esophageal squamous epithelium.
Genom. Data 12:4-6(2017)

Cross-references
Cell line collections (Providers) ATCC; CRL-2692
Cell line databases/resources CLO; CLO_0003718
Anatomy/cell type resources BTO; BTO_0004433
Biological sample resources BioSample; SAMN03471766
Chemistry resources ChEMBL-Cells; CHEMBL4295465
ChEMBL-Targets; CHEMBL4296426
PubChem_Cell_line; CVCL_3702
Encyclopedic resources Wikidata; Q54882925
Experimental variables resources EFO; EFO_0022693
Gene expression databases GEO; GSM1375723
GEO; GSM1375724
GEO; GSM1375725
GEO; GSM1375726
GEO; GSM1375735
GEO; GSM1375736
GEO; GSM1375737
GEO; GSM1375738
GEO; GSM1375747
GEO; GSM1375748
GEO; GSM1375749
GEO; GSM1375750
GEO; GSM1508954
GEO; GSM1508958
Polymorphism and mutation databases IARC_TP53; 21630
IARC_TP53; 23555
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number25