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Cellosaurus MT-3 [Human contaminated breast cancer] (CVCL_2129)

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Cell line name MT-3 [Human contaminated breast cancer]
Synonyms MT3
Accession CVCL_2129
Resource Identification Initiative To cite this cell line use: MT-3 [Human contaminated breast cancer] (RRID:CVCL_2129)
Comments Problematic cell line: Contaminated. Shown to be a LS174T derivative (PubMed=25877200; DSMZ). Originally thought to originate from a breast carcinoma.
Part of: MD Anderson Cell Lines Project.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00506.
Population: Caucasian.
Doubling time: 37.1 +- 8.1 hours (PubMed=9150904); ~30 hours (DSMZ=ACC-403).
Microsatellite instability: Instable (MSI) (PubMed=12661003; PubMed=15677628).
Omics: Deep exome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Cell type: Epithelial cell of colon; CL=CL_0011108.
Sequence variations
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45Phe (c.134C>T); ClinVar=VCV000017588; Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Unspecified (from parent cell line).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:01,02:01
HLA-BB*07:17,15:50
HLA-CC*04:01,06:02
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1384 (LS174T)
Sex of cell Female
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-403; PubMed=25877200

Markers:
AmelogeninX
CSF1PO10,13
D2S133818,22
D3S135815,16
D5S81812,17 (DSMZ=ACC-403)
12 (PubMed=25877200)
D7S82010.3,11,12
D8S117912,15 (PubMed=25877200)
13,15 (DSMZ=ACC-403)
D13S31710,11
D16S53911,13
D18S5110,12
D19S43313,14
D21S1129,31
FGA20,21
Penta D9,10
Penta E13,15 (PubMed=25877200)
13,15,17,21 (DSMZ=ACC-403)
TH016,7
TPOX8,9
vWA13,14,16,17 (DSMZ=ACC-403)
14,17 (PubMed=25877200)

Run an STR similarity search on this cell line
Web pages https://www.cellosaurus.org/pawefish/BreastCellLineDescriptions/MT-3.html
https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
https://tcpaportal.org/mclp/
Publications

PubMed=1446057; DOI=10.1007/BF01831480
Naundorf H., Rewasowa E.C., Fichtner I., Buttner B., Becker M., Gorlich M.
Characterization of two human mammary carcinomas, MT-1 and MT-3, suitable for in vivo testing of ether lipids and their derivatives.
Breast Cancer Res. Treat. 23:87-95(1992)

PubMed=9150904; DOI=10.1023/A:1005756632293
Hambly R.J., Double J.A., Thompson M.J., Bibby M.C.
Establishment and characterisation of new cell lines from human breast tumours initially established as tumour xenografts in NMRI nude mice.
Breast Cancer Res. Treat. 43:247-258(1997)

PubMed=11044355; DOI=10.1054/bjoc.2000.1458; PMCID=PMC2408781
Davidson J.M., Gorringe K.L., Chin S.-F., Orsetti B., Besret C., Courtay-Cahen C., Roberts I., Theillet C., Caldas C., Edwards P.A.W.
Molecular cytogenetic analysis of breast cancer cell lines.
Br. J. Cancer 83:1309-1317(2000)

PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=12800145; DOI=10.1002/gcc.10218
Adelaide J., Huang H.-E., Murati A., Alsop A.E., Orsetti B., Mozziconacci M.-J., Popovici C., Ginestier C., Letessier A., Basset C., Courtay-Cahen C., Jacquemier J., Theillet C., Birnbaum D., Edwards P.A.W., Chaffanet M.
A recurrent chromosome translocation breakpoint in breast and pancreatic cancer cell lines targets the neuregulin/NRG1 gene.
Genes Chromosomes Cancer 37:333-345(2003)

PubMed=15677628; DOI=10.1093/carcin/bgi032
Gorringe K.L., Chin S.-F., Pharoah P.D.P., Staines J.M., Oliveira C., Edwards P.A.W., Caldas C.
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110; PMCID=PMC3937590
Daemen A., Griffith O.L., Heiser L.M., Wang N.J., Enache O.M., Sanborn Z., Pepin F., Durinck S., Korkola J.E., Griffith M., Hur J.S., Huh N., Chung J., Cope L., Fackler M.J., Umbricht C.B., Sukumar S., Seth P., Sukhatme V.P., Jakkula L.R., Lu Y.-L., Mills G.B., Cho R.J., Collisson E.A., van 't Veer L.J., Spellman P.T., Gray J.W.
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections (Providers) DSMZ; ACC-403 - Discontinued
Cell line databases/resources CLO; CLO_0007890
cancercelllines; CVCL_2129
DSMZCellDive; ACC-403
IGRhCellID; MT3
LINCS_HMS; 50031
LINCS_LDP; LCL-1473
Biological sample resources BioSample; SAMN03472975
BioSample; SAMN03473582
BioSample; SAMN06481118
Chemistry resources PharmacoDB; MT3_969_2019
Encyclopedic resources Wikidata; Q54906937
Experimental variables resources EFO; EFO_0002241
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2706
GEO; GSM115102
GEO; GSM217592
GEO; GSM412073
GEO; GSM412084
GEO; GSM412093
GEO; GSM412098
GEO; GSM412106
GEO; GSM412110
GEO; GSM459700
GEO; GSM474280
GEO; GSM474281
GEO; GSM476484
GEO; GSM476485
GEO; GSM478311
GEO; GSM478312
GEO; GSM478314
GEO; GSM478315
GEO; GSM478320
GEO; GSM478321
GEO; GSM783977
GEO; GSM847419
GEO; GSM847498
GEO; GSM844610
GEO; GSM1172986
Polymorphism and mutation databases Progenetix; CVCL_2129
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number34