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Cellosaurus ECV-304 (CVCL_2029)

[Text version]
Cell line name ECV-304
Synonyms ECV 304; ECV304; ECV; E304; T24(ECV304)
Accession CVCL_2029
Resource Identification Initiative To cite this cell line use: ECV-304 (RRID:CVCL_2029)
Comments Problematic cell line: Contaminated. Shown to be a T24 derivative (DOI=10.11418/jtca1981.18.4_329; PubMed=10508494; PubMed=10614862; PubMed=12197766; PubMed=20143388). Originally thought to originate from endothelial cells obtained from the umbilical vein of a healthy donor.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00117.
Population: Caucasian; Swedish.
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 35 hours (PubMed=1817644); ~2 days (DSMZ=ACC-310).
Omics: Deep tyrosine phosphoproteome analysis.
Miscellaneous: Formerly the CCRID database had 2 entries describing this cell line (3111C0002000000074, 3142C0001000000023). They were one of the sources for the STR profile of this entry.
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
Sequence variations
  • Mutation; HGNC; 5173; HRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012600; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0554 (T24)
CVCL_KS70 (CellSensor IL-1-bla ECV304)CVCL_UY91 (t-HUE1)CVCL_J427 (t-HUE2)
Sex of cell Female
Age at sampling 82Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS; DSMZ; ECACC; JCRB; PubMed=11416159

D8S117910,14 (DSMZ)
14 (CLS; PubMed=11416159)
Penta D11,15
Penta E7,10

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/e/cell-lines-detail-2.html

PubMed=1690702; DOI=10.1007/BF02624456
Takahashi K., Sawasaki Y., Hata J.-i., Mukai K., Goto T.
Spontaneous transformation and immortalization of human endothelial cells.
In Vitro Cell. Dev. Biol. 26:265-274(1990)

Kobayashi M., Kondo M., Mitsui Y.
Establishment of human endothelial cell lines in a serum-free culture and its application for expression of transfected prepro endothelin gene.
Hum. Cell 4:296-305(1991)

PubMed=1378828; DOI=10.1007/BF02634037
Takahasi K., Sawasaki Y.
Rare spontaneously transformed human endothelial cell line provides useful research tool.
In Vitro Cell. Dev. Biol. Anim. 28:380-382(1992)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

Tanabe H., Takada Y., Minegishi D., Kurematsu M., Masui T., Mizusawa H.
Cell line individualization by STR multiplex system in the cell bank found cross-contamination between ECV304 and EJ-1/T24.
Tissue Cult. Res. Commun. 18:329-338(1999)

PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2
MacLeod R.A.F., Dirks W.G., Matsuo Y., Kaufmann M., Milch H., Drexler H.G.
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Int. J. Cancer 83:555-563(1999)

PubMed=10614862; DOI=10.1007/s11626-999-0091-8
Dirks W.G., MacLeod R.A.F., Drexler H.G.
ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross-contamination at source.
In Vitro Cell. Dev. Biol. Anim. 35:558-559(1999)

PubMed=10653001; DOI=10.1038/labinvest.3780006
Brown J., Reading S.J., Jones S., Fitchett C.J., Howl J., Martin A., Longland C.L., Michelangeli F., Dubrova Y.E., Brown C.A.
Critical evaluation of ECV304 as a human endothelial cell model defined by genetic analysis and functional responses: a comparison with the human bladder cancer derived epithelial cell line T24/83.
Lab. Invest. 80:37-45(2000)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11669284; DOI=10.1290/1071-2690(2001)037<0505:PCOHUV>2.0.CO;2
Suda K., Rothen-Rutishauser B., Gunthert M., Wunderli-Allenspach H.
Phenotypic characterization of human umbilical vein endothelial (ECV304) and urinary carcinoma (T24) cells: endothelial versus epithelial features.
In Vitro Cell. Dev. Biol. Anim. 37:505-514(2001)

PubMed=12197766; DOI=10.1290/1071-2690(2002)038<0185:BCCLEI>2.0.CO;2
Drexler H.G., Quentmeier H., Dirks W.G., MacLeod R.A.F.
Bladder carcinoma cell line ECV304 is not a model system for endothelial cells.
In Vitro Cell. Dev. Biol. Anim. 38:185-186(2002)

PubMed=12453433; DOI=10.1006/mvre.2002.2434
Unger R.E., Krump-Konvalinkova V., Peters K., Kirkpatrick C.J.
In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.
Microvasc. Res. 64:384-397(2002)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=27554326; DOI=10.1038/srep31811
Xie Y.-J., Wang J.-L., Zhang Y.-Y., Liu X.-F., Wang X.-R., Liu K.-H., Huang X.-H., Wang Y.-C.
Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells.
Sci. Rep. 6:31811-31811(2016)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cell line collections (Providers) ATCC; CRL-1998 - Discontinued
BCRJ; 0283 - Discontinued
CLS; 300452
DSMZ; ACC-310 - Discontinued
ECACC; 92091712
JCRB; NIHS0220 - Discontinued
Cell line databases/resources CLO; CLO_0002881
CLO; CLO_0002882
CLDB; cl1136
CLDB; cl5185
cancercelllines; CVCL_2029
DSMZCellDive; ACC-310
Anatomy/cell type resources BTO; BTO:0002043
Biological sample resources BioSample; SAMN03151937
BioSample; SAMN03471274
Chemistry resources ChEMBL-Cells; CHEMBL3307513
ChEMBL-Targets; CHEMBL614035
PubChem_Cell_line; CVCL_2029
Encyclopedic resources Wikidata; Q54831948
Polymorphism and mutation databases Cosmic; 1466801
Proteomic databases PRIDE; PXD004340
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number34