ID   ECV-304
AC   CVCL_2029
SY   ECV 304; ECV304; ECV; E304; T24(ECV304)
DR   BTO; BTO:0002043
DR   CLO; CLO_0002881
DR   CLO; CLO_0002882
DR   CLDB; cl1136
DR   CLDB; cl5185
DR   ATCC; CRL-1998
DR   BCRJ; 0283
DR   BioSample; SAMN03151937
DR   BioSample; SAMN03471274
DR   cancercelllines; CVCL_2029
DR   ChEMBL-Cells; CHEMBL3307513
DR   ChEMBL-Targets; CHEMBL614035
DR   CLS; 300452
DR   Cosmic; 1466801
DR   DSMZ; ACC-310
DR   DSMZCellDive; ACC-310
DR   ECACC; 92091712
DR   IZSLER; BS CL 137
DR   JCRB; JCRB0744
DR   JCRB; NIHS0220
DR   PRIDE; PXD004340
DR   PubChem_Cell_line; CVCL_2029
DR   Wikidata; Q54831948
RX   DOI=10.11418/jtca1981.18.4_329;
RX   PubMed=1378828;
RX   PubMed=1690702;
RX   PubMed=1817644;
RX   PubMed=9290701;
RX   PubMed=10508494;
RX   PubMed=10614862;
RX   PubMed=10653001;
RX   PubMed=11416159;
RX   PubMed=11669284;
RX   PubMed=12197766;
RX   PubMed=12453433;
RX   PubMed=20143388;
RX   PubMed=27554326;
RX   PubMed=33389257;
WW   https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/e/cell-lines-detail-2.html
WW   https://www.atcc.org/en/support/technical-support/faqs/ecv-304-atcc-crl-1998
WW   https://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
CC   Problematic cell line: Contaminated. Shown to be a T24 derivative (DOI=10.11418/jtca1981.18.4_329; PubMed=10508494; PubMed=10614862; PubMed=12197766; PubMed=20143388). Originally thought to originate from endothelial cells obtained from the umbilical vein of a healthy donor.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00117.
CC   Population: Caucasian; Swedish.
CC   Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
CC   Doubling time: 35 hours (PubMed=1817644); ~2 days (DSMZ=ACC-310).
CC   Sequence variation: Mutation; HGNC; 5173; HRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012600; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
CC   Omics: Deep tyrosine phosphoproteome analysis.
CC   Miscellaneous: Formerly the CCRID database had 2 entries describing this cell line (3111C0002000000074, 3142C0001000000023). They were one of the sources for the STR profile of this entry.
CC   Discontinued: ATCC; CRL-1998; true.
CC   Discontinued: BCRJ; 0283; probable.
CC   Discontinued: DSMZ; ACC-310; true.
CC   Discontinued: JCRB; NIHS0220; true.
CC   Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
ST   Source(s): ATCC; CCRID; CLS; DSMZ; ECACC; JCRB; PubMed=11416159
ST   Amelogenin: X
ST   CSF1PO: 12
ST   D13S317: 12
ST   D16S539: 9
ST   D18S51: 16,18
ST   D19S433: 13,14
ST   D21S11: 29
ST   D2S1338: 20,23
ST   D3S1358: 16
ST   D5S818: 10
ST   D7S820: 10,11
ST   D8S1179: 10,14 (DSMZ)
ST   D8S1179: 14 (CLS; PubMed=11416159)
ST   FGA: 17,22
ST   Penta D: 11,15
ST   Penta E: 7,10
ST   TH01: 6
ST   TPOX: 8,11
ST   vWA: 17
DI   NCIt; C4912; Bladder carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0554 ! T24
SX   Female
AG   82Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 34
//
RX   DOI=10.11418/jtca1981.18.4_329;
RA   Tanabe H., Takada Y., Minegishi D., Kurematsu M., Masui T.,
RA   Mizusawa H.;
RT   "Cell line individualization by STR multiplex system in the cell bank
RT   found cross-contamination between ECV304 and EJ-1/T24.";
RL   Tissue Cult. Res. Commun. 18:329-338(1999).
//
RX   PubMed=1378828; DOI=10.1007/BF02634037;
RA   Takahasi K., Sawasaki Y.;
RT   "Rare spontaneously transformed human endothelial cell line provides
RT   useful research tool.";
RL   In Vitro Cell. Dev. Biol. Anim. 28:380-382(1992).
//
RX   PubMed=1690702; DOI=10.1007/BF02624456;
RA   Takahashi K., Sawasaki Y., Hata J.-i., Mukai K., Goto T.;
RT   "Spontaneous transformation and immortalization of human endothelial
RT   cells.";
RL   In Vitro Cell. Dev. Biol. 26:265-274(1990).
//
RX   PubMed=1817644;
RA   Kobayashi M., Kondo M., Mitsui Y.;
RT   "Establishment of human endothelial cell lines in a serum-free culture
RT   and its application for expression of transfected prepro endothelin
RT   gene.";
RL   Hum. Cell 4:296-305(1991).
//
RX   PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D;
RA   Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T.,
RA   Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M.,
RA   Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.;
RT   "Screening the p53 status of human cell lines using a yeast functional
RT   assay.";
RL   Mol. Carcinog. 19:243-253(1997).
//
RX   PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2;
RA   MacLeod R.A.F., Dirks W.G., Matsuo Y., Kaufmann M., Milch H.,
RA   Drexler H.G.;
RT   "Widespread intraspecies cross-contamination of human tumor cell lines
RT   arising at source.";
RL   Int. J. Cancer 83:555-563(1999).
//
RX   PubMed=10614862; DOI=10.1007/s11626-999-0091-8;
RA   Dirks W.G., MacLeod R.A.F., Drexler H.G.;
RT   "ECV304 (endothelial) is really T24 (bladder carcinoma): cell line
RT   cross-contamination at source.";
RL   In Vitro Cell. Dev. Biol. Anim. 35:558-559(1999).
//
RX   PubMed=10653001; DOI=10.1038/labinvest.3780006;
RA   Brown J., Reading S.J., Jones S., Fitchett C.J., Howl J., Martin A.,
RA   Longland C.L., Michelangeli F., Dubrova Y.E., Brown C.A.;
RT   "Critical evaluation of ECV304 as a human endothelial cell model
RT   defined by genetic analysis and functional responses: a comparison
RT   with the human bladder cancer derived epithelial cell line T24/83.";
RL   Lab. Invest. 80:37-45(2000).
//
RX   PubMed=11416159; DOI=10.1073/pnas.121616198;
RA   Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G.,
RA   Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R.,
RA   Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.;
RT   "Short tandem repeat profiling provides an international reference
RT   standard for human cell lines.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).
//
RX   PubMed=11669284; DOI=10.1290/1071-2690(2001)037<0505:PCOHUV>2.0.CO;2;
RA   Suda K., Rothen-Rutishauser B., Gunthert M., Wunderli-Allenspach H.;
RT   "Phenotypic characterization of human umbilical vein endothelial
RT   (ECV304) and urinary carcinoma (T24) cells: endothelial versus
RT   epithelial features.";
RL   In Vitro Cell. Dev. Biol. Anim. 37:505-514(2001).
//
RX   PubMed=12197766; DOI=10.1290/1071-2690(2002)038<0185:BCCLEI>2.0.CO;2;
RA   Drexler H.G., Quentmeier H., Dirks W.G., MacLeod R.A.F.;
RT   "Bladder carcinoma cell line ECV304 is not a model system for
RT   endothelial cells.";
RL   In Vitro Cell. Dev. Biol. Anim. 38:185-186(2002).
//
RX   PubMed=12453433; DOI=10.1006/mvre.2002.2434;
RA   Unger R.E., Krump-Konvalinkova V., Peters K., Kirkpatrick C.J.;
RT   "In vitro expression of the endothelial phenotype: comparative study
RT   of primary isolated cells and cell lines, including the novel cell
RT   line HPMEC-ST1.6R.";
RL   Microvasc. Res. 64:384-397(2002).
//
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A.,
RA   MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R.,
RA   Freshney R.I.;
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
//
RX   PubMed=27554326; DOI=10.1038/srep31811;
RA   Xie Y.-J., Wang J.-L., Zhang Y.-Y., Liu X.-F., Wang X.-R.,
RA   Liu K.-H., Huang X.-H., Wang Y.-C.;
RT   "Quantitative profiling of spreading-coupled protein tyrosine
RT   phosphorylation in migratory cells.";
RL   Sci. Rep. 6:31811-31811(2016).
//
RX   PubMed=33389257; DOI=10.1007/s10096-020-04106-0;
RA   Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.;
RT   "Culture of SARS-CoV-2 in a panel of laboratory cell lines,
RT   permissivity, and differences in growth profile.";
RL   Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021).
//