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Cellosaurus P39 (CVCL_0478)

[Text version]
Cell line name P39
Synonyms P39/TSU; P39-TSU; P39 TSU; P39TSU; P-39:TSU; P39/Tsugane; P39/TSUGANE; TSUGANE; Tsugane
Accession CVCL_0478
Resource Identification Initiative To cite this cell line use: P39 (RRID:CVCL_0478)
Comments Problematic cell line: Contaminated. Shown to be a HL-60 derivative (PubMed=12592342; PubMed=20143388; PubMed=20421273). Originally thought to originate from the peripheral blood of a 69 year old male with acute myeloid leukemia.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00255.
Population: Caucasian.
Microsatellite instability: Stable (MSS) (PubMed=10739008; PubMed=11226526).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene deletion; HGNC; 11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (PubMed=9379676).
Disease Adult acute myeloid leukemia (NCIt: C9154)
Acute myeloid leukemia (ORDO: Orphanet_519)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0002 (HL-60)
Sex of cell Female
Age at sampling 36Y
Category Cancer cell line
STR profile Source(s): JCRB

Markers:
AmelogeninX
CSF1PO13,14
D5S81811,12
D7S82011,12
D13S3178,11
D16S53911
TH017,8
TPOX8,11
vWA15,16

Run an STR similarity search on this cell line
Web pages https://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=6597119; DOI=10.20772/cancersci1959.75.12_1100
Nagai M., Seki S., Kitahara T., Abe T., Minato K., Watanabe S., Shimoyama M.
A novel human myelomonocytoid cell line, P39/Tsugane, derived from overt leukemia following myelodysplastic syndrome.
Gann 75:1100-1107(1984)

PubMed=9379676; DOI=10.1016/S0145-2126(97)00036-2
Sheng X.-M., Kawamura M., Ohnishi H., Ida K., Hanada R., Kojima S., Kobayashi M., Bessho F., Yanagisawa M., Hayashi Y.
Mutations of the RAS genes in childhood acute myeloid leukemia, myelodysplastic syndrome and juvenile chronic myelocytic leukemia.
Leuk. Res. 21:697-701(1997)

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Leuk. Res. 24:255-262(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000),255-262.
Leuk. Res. 25:275-278(2001)

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20421273; DOI=10.3324/haematol.2010.022988
Steensma D.P.
P39/Tsugane cells are a false cell line contaminated with HL-60 cells and are not suitable for mechanistic studies in myelodysplastic syndromes.
Haematologica 95:1229-1230(2010)

PubMed=23955599; DOI=10.1038/ng.2731
Kon A., Shih L.-Y., Minamino M., Sanada M., Shiraishi Y., Nagata Y., Yoshida K.-i., Okuno Y., Bando M., Nakato R., Ishikawa S., Sato-Otsubo A., Nagae G., Nishimoto A., Haferlach C., Nowak D., Sato Y., Alpermann T., Nagasaki M., Shimamura T., Tanaka H., Chiba K., Yamamoto R., Yamaguchi T., Otsu M., Obara N., Sakata-Yanagimoto M., Nakamaki T., Ishiyama K., Nolte F., Hofmann W.-K., Miyawaki S., Chiba S., Mori H., Nakauchi H., Koeffler H.P., Aburatani H., Haferlach T., Shirahige K., Miyano S., Ogawa S.
Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.
Nat. Genet. 45:1232-1237(2013)

Cross-references
Cell line collections (Providers) JCRB; JCRB0092
Cell line databases/resources MCCL; MCC:0000376
cancercelllines; CVCL_0478
Biological sample resources BioSample; SAMN03151775
BioSample; SAMN03471068
Encyclopedic resources Wikidata; Q54937278
Polymorphism and mutation databases Cosmic; 717681
Cosmic; 947362
Cosmic; 998740
Cosmic; 2089663
IARC_TP53; 21275
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number32