ID   P39
AC   CVCL_0478
SY   P39/TSU; P39-TSU; P39 TSU; P39TSU; P-39:TSU; P39/Tsugane; P39/TSUGANE; TSUGANE; Tsugane
DR   MCCL; MCC:0000376
DR   BioSample; SAMN03151775
DR   BioSample; SAMN03471068
DR   cancercelllines; CVCL_0478
DR   Cosmic; 717681
DR   Cosmic; 947362
DR   Cosmic; 998740
DR   Cosmic; 2089663
DR   IARC_TP53; 21275
DR   JCRB; JCRB0092
DR   Wikidata; Q54937278
RX   DOI=10.1016/B978-0-12-221970-2.50457-5;
RX   PubMed=6597119;
RX   PubMed=9379676;
RX   PubMed=10739008;
RX   PubMed=11226526;
RX   PubMed=12592342;
RX   PubMed=20143388;
RX   PubMed=20421273;
RX   PubMed=23955599;
WW   https://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
CC   Problematic cell line: Contaminated. Shown to be a HL-60 derivative (PubMed=12592342; PubMed=20143388; PubMed=20421273). Originally thought to originate from the peripheral blood of a 69 year old male with acute myeloid leukemia.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00255.
CC   Population: Caucasian.
CC   Microsatellite instability: Stable (MSS) (PubMed=10739008; PubMed=11226526).
CC   Sequence variation: Gene deletion; HGNC; 11998; TP53; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (PubMed=9379676).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
ST   Source(s): JCRB
ST   Amelogenin: X
ST   CSF1PO: 13,14
ST   D13S317: 8,11
ST   D16S539: 11
ST   D5S818: 11,12
ST   D7S820: 11,12
ST   TH01: 7,8
ST   TPOX: 8,11
ST   vWA: 15,16
DI   NCIt; C9154; Adult acute myeloid leukemia
DI   ORDO; Orphanet_519; Acute myeloid leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0002 ! HL-60
SX   Female
AG   36Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 32
//
RX   DOI=10.1016/B978-0-12-221970-2.50457-5;
RA   Drexler H.G.;
RT   "The leukemia-lymphoma cell line factsbook.";
RL   (In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001).
//
RX   PubMed=6597119; DOI=10.20772/cancersci1959.75.12_1100;
RA   Nagai M., Seki S., Kitahara T., Abe T., Minato K., Watanabe S.,
RA   Shimoyama M.;
RT   "A novel human myelomonocytoid cell line, P39/Tsugane, derived from
RT   overt leukemia following myelodysplastic syndrome.";
RL   Gann 75:1100-1107(1984).
//
RX   PubMed=9379676; DOI=10.1016/S0145-2126(97)00036-2;
RA   Sheng X.-M., Kawamura M., Ohnishi H., Ida K., Hanada R., Kojima S.,
RA   Kobayashi M., Bessho F., Yanagisawa M., Hayashi Y.;
RT   "Mutations of the RAS genes in childhood acute myeloid leukemia,
RT   myelodysplastic syndrome and juvenile chronic myelocytic leukemia.";
RL   Leuk. Res. 21:697-701(1997).
//
RX   PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4;
RA   Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.;
RT   "Frequent microsatellite instability and BAX mutations in T cell acute
RT   lymphoblastic leukemia cell lines.";
RL   Leuk. Res. 24:255-262(2000).
//
RX   PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1;
RA   Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.;
RT   "Corrigendum to: Frequent microsatellite instability and BAX mutations
RT   in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24
RT   (2000),255-262.";
RL   Leuk. Res. 25:275-278(2001).
//
RX   PubMed=12592342; DOI=10.1038/sj.leu.2402799;
RA   Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.;
RT   "False leukemia-lymphoma cell lines: an update on over 500 cell
RT   lines.";
RL   Leukemia 17:416-426(2003).
//
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A.,
RA   MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R.,
RA   Freshney R.I.;
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
//
RX   PubMed=20421273; DOI=10.3324/haematol.2010.022988;
RA   Steensma D.P.;
RT   "P39/Tsugane cells are a false cell line contaminated with HL-60 cells
RT   and are not suitable for mechanistic studies in myelodysplastic
RT   syndromes.";
RL   Haematologica 95:1229-1230(2010).
//
RX   PubMed=23955599; DOI=10.1038/ng.2731;
RA   Kon A., Shih L.-Y., Minamino M., Sanada M., Shiraishi Y., Nagata Y.,
RA   Yoshida K.-i., Okuno Y., Bando M., Nakato R., Ishikawa S.,
RA   Sato-Otsubo A., Nagae G., Nishimoto A., Haferlach C., Nowak D.,
RA   Sato Y., Alpermann T., Nagasaki M., Shimamura T., Tanaka H., Chiba K.,
RA   Yamamoto R., Yamaguchi T., Otsu M., Obara N., Sakata-Yanagimoto M.,
RA   Nakamaki T., Ishiyama K., Nolte F., Hofmann W.-K., Miyawaki S.,
RA   Chiba S., Mori H., Nakauchi H., Koeffler H.P., Aburatani H.,
RA   Haferlach T., Shirahige K., Miyano S., Ogawa S.;
RT   "Recurrent mutations in multiple components of the cohesin complex in
RT   myeloid neoplasms.";
RL   Nat. Genet. 45:1232-1237(2013).
//