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Cellosaurus J.CaM1.6 (CVCL_0354)

[Text version]
Cell line name J.CaM1.6
Synonyms J CaM 1.6; J-CaM-1.6; JCam-1.6; JCaM 1.6; JCAM 1.6; JCaM1.6; Jcam clone 1.6; J.CaM1; JCaM-1; JCaM1; JCAM1; Jurkat-derived Ca2+ mutant no. 1
Accession CVCL_0354
Resource Identification Initiative To cite this cell line use: J.CaM1.6 (RRID:CVCL_0354)
Comments Population: Caucasian.
Characteristics: Deficient for LCK activity due to a deletion of exon 7.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (PubMed=7592872).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0367 (Jurkat E6.1)
Children:
CVCL_B7TT (J.CaM1.7)CVCL_XF41 (JCaM1/TAg)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2063; ECACC=96060401

Markers:
AmelogeninX,Y
CSF1PO11
D2S133819,23
D3S135815,17
D5S8189
D7S8208,10
D8S117913,14
D13S3178,12
D16S53910,11
D18S5113,22
D19S43314.2,15
D21S1131.2,33.2
FGA20,21
Penta D11,13
Penta E10,11
TH016,9.3
TPOX8,10
vWA18,19

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/j/cell-lines-detail-80.html
Publications

PubMed=3309950; DOI=10.1073/pnas.84.19.6879; PMCID=PMC299188
Goldsmith M.A., Weiss A.
Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor.
Proc. Natl. Acad. Sci. U.S.A. 84:6879-6883(1987)

PubMed=3263650; DOI=10.1073/pnas.85.22.8613; PMCID=PMC282509
Goldsmith M.A., Dazin P.F., Weiss A.
At least two non-antigen-binding molecules are required for signal transduction by the T-cell antigen receptor.
Proc. Natl. Acad. Sci. U.S.A. 85:8613-8617(1988)

PubMed=7592872; DOI=10.1074/jbc.270.44.26533
Fargnoli J., Burkhardt A.L., Laverty M., Kut S.A., van Oers N.S.C., Weiss A., Bolen J.B.
Syk mutation in Jurkat E6-derived clones results in lack of p72syk expression.
J. Biol. Chem. 270:26533-26537(1995)

PubMed=8626636; DOI=10.1074/jbc.271.17.9976
Straus D.B., Chan A.C.-Y., Patai B., Weiss A.
SH2 domain function is essential for the role of the Lck tyrosine kinase in T cell receptor signal transduction.
J. Biol. Chem. 271:9976-9981(1996)

PubMed=11231305; DOI=10.1046/j.1432-1327.2001.02028.x
Livolsi A., Busuttil V., Imbert V., Abraham R.T., Peyron J.-F.
Tyrosine phosphorylation-dependent activation of NF-kappa B Requirement for p56 LCK and ZAP-70 protein tyrosine kinases.
Eur. J. Biochem. 268:1508-1515(2001)

PubMed=33562083; DOI=10.3390/cells10020343; PMCID=PMC7915312
Vico-Barranco I., de Arbulo-Echevarria M.M., Serrano-Garcia I., Perez-Linaza A., Miranda-Sayago J.M., Miazek A., Narbona-Sanchez I., Aguado E.
A novel, LAT/Lck double deficient T cell subline J.CaM1.7 for combined analysis of early TCR signaling.
Cells 10:343.1-343.20(2021)

Cross-references
Cell line collections (Providers) ATCC; CRL-2063
ECACC; 96060401
Cell line databases/resources CLO; CLO_0006983
MCCL; MCC:0000248
CLDB; cl2934
cancercelllines; CVCL_0354
Anatomy/cell type resources BTO; BTO:0003314
Biological sample resources BioSample; SAMN03471427
Encyclopedic resources Wikidata; Q54898427
Experimental variables resources EFO; EFO_0022656
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number29