Due to maintenance work, this service will be unavailable from
Mon Nov 11 17:30 until
Tue Nov 12 09:00
CET.
Apologies for the inconvenience.
Cell line name |
J.CaM1.6 |
Synonyms |
J CaM 1.6; J-CaM-1.6; JCam-1.6; JCaM 1.6; JCAM 1.6; JCaM1.6; Jcam clone 1.6; J.CaM1; JCaM-1; JCaM1; JCAM1; Jurkat-derived Ca2+ mutant no. 1 |
Accession |
CVCL_0354 |
Resource Identification Initiative |
To cite this cell line use: J.CaM1.6 (RRID:CVCL_0354) |
Comments |
Population: Caucasian. Characteristics: Deficient for LCK activity due to a deletion of exon 7. Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178. Cell type: T-cell; CL=CL_0000084. |
Sequence variations |
- Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (PubMed=7592872).
- Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
|
Disease |
Childhood T acute lymphoblastic leukemia (NCIt: C7953) Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861) |
Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
Hierarchy |
Parent: CVCL_0367 (Jurkat E6.1) Children:
|
Sex of cell |
Male |
Age at sampling |
14Y |
Category |
Cancer cell line |
STR profile |
Source(s): ATCC=CRL-2063; ECACC=96060401
Markers:Amelogenin | X,Y |
CSF1PO | 11 |
D2S1338 | 19,23 |
D3S1358 | 15,17 |
D5S818 | 9 |
D7S820 | 8,10 |
D8S1179 | 13,14 |
D13S317 | 8,12 |
D16S539 | 10,11 |
D18S51 | 13,22 |
D19S433 | 14.2,15 |
D21S11 | 31.2,33.2 |
FGA | 20,21 |
Penta D | 11,13 |
Penta E | 10,11 |
TH01 | 6,9.3 |
TPOX | 8,10 |
vWA | 18,19 |
Run an STR similarity search on this cell line |
Web pages |
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/j/cell-lines-detail-80.html |
Publications | PubMed=3309950; DOI=10.1073/pnas.84.19.6879; PMCID=PMC299188 Goldsmith M.A., Weiss A. Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor. Proc. Natl. Acad. Sci. U.S.A. 84:6879-6883(1987) PubMed=3263650; DOI=10.1073/pnas.85.22.8613; PMCID=PMC282509 Goldsmith M.A., Dazin P.F., Weiss A. At least two non-antigen-binding molecules are required for signal transduction by the T-cell antigen receptor. Proc. Natl. Acad. Sci. U.S.A. 85:8613-8617(1988) PubMed=7592872; DOI=10.1074/jbc.270.44.26533 Fargnoli J., Burkhardt A.L., Laverty M., Kut S.A., van Oers N.S.C., Weiss A., Bolen J.B. Syk mutation in Jurkat E6-derived clones results in lack of p72syk expression. J. Biol. Chem. 270:26533-26537(1995) PubMed=8626636; DOI=10.1074/jbc.271.17.9976 Straus D.B., Chan A.C.-Y., Patai B., Weiss A. SH2 domain function is essential for the role of the Lck tyrosine kinase in T cell receptor signal transduction. J. Biol. Chem. 271:9976-9981(1996) PubMed=11231305; DOI=10.1046/j.1432-1327.2001.02028.x Livolsi A., Busuttil V., Imbert V., Abraham R.T., Peyron J.-F. Tyrosine phosphorylation-dependent activation of NF-kappa B Requirement for p56 LCK and ZAP-70 protein tyrosine kinases. Eur. J. Biochem. 268:1508-1515(2001) PubMed=33562083; DOI=10.3390/cells10020343; PMCID=PMC7915312 Vico-Barranco I., de Arbulo-Echevarria M.M., Serrano-Garcia I., Perez-Linaza A., Miranda-Sayago J.M., Miazek A., Narbona-Sanchez I., Aguado E. A novel, LAT/Lck double deficient T cell subline J.CaM1.7 for combined analysis of early TCR signaling. Cells 10:343.1-343.20(2021) |
Cross-references |
Cell line collections (Providers) |
ATCC; CRL-2063
ECACC; 96060401
|
Cell line databases/resources |
CLO; CLO_0006983
MCCL; MCC:0000248
CLDB; cl2934
cancercelllines; CVCL_0354
|
Anatomy/cell type resources |
BTO; BTO:0003314
|
Biological sample resources |
BioSample; SAMN03471427
|
Encyclopedic resources |
Wikidata; Q54898427
|
Experimental variables resources |
EFO; EFO_0022656
|
Entry history |
Entry creation | 04-Apr-2012 |
Last entry update | 10-Sep-2024 |
Version number | 29 |
---|