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Cellosaurus V79/E36-ts20 (CVCL_0215)

[Text version]
Cell line name V79/E36-ts20
Synonyms E36-ts20; E36ts20; TS20; ts20; ts 20
Accession CVCL_0215
Resource Identification Initiative To cite this cell line use: V79/E36-ts20 (RRID:CVCL_0215)
Comments Characteristics: Has a thermolabile ubiquitin-like modifier-activating enzyme 1 (Uba1) (PubMed=3049611).
Selected for resistance to: ChEBI; CHEBI:63486; 8-azaguanine.
Misspelling: CHO-TS20; Note=Incorrect as not a CHO derivative but a V79 derivative.
Misspelling: CHO-ts20; Note=Incorrect as not a CHO derivative but a V79 derivative.
Misspelling: CHO ts20; Note=Incorrect as not a CHO derivative but a V79 derivative.
Misspelling: CHOTS20; Note=Incorrect as not a CHO derivative but a V79 derivative.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Cell type: Fibroblast of lung; CL=CL_0002553.
Sequence variations
  • Mutation; UniProtKB; O09185; Tp53; Simple; p.Leu133Gln (c.398T>A); Zygosity=Unspecified (from parent cell line).
  • Mutation; UniProtKB; O09185; Tp53; Simple; p.Cys135Trp (c.405C>G); Zygosity=Unspecified (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_5A58 (V79/E36)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=6752155; DOI=10.1002/jcp.1041130125
Hirschberg J., Marcus M.
Isolation by a replica-plating technique of Chinese hamster temperature-sensitive cell cycle mutants.
J. Cell. Physiol. 113:159-166(1982)

PubMed=3049611; DOI=10.1016/S0021-9258(19)37648-3
Kulka R.G., Raboy B., Schuster R., Parag H.A., Diamond G., Ciechanover A., Marcus M.
A Chinese hamster cell cycle mutant arrested at G2 phase has a temperature-sensitive ubiquitin-activating enzyme, E1.
J. Biol. Chem. 263:15726-15731(1988)

Cross-references
Cell line databases/resources MCCL; MCC:0000104
Encyclopedic resources Wikidata; Q54992616
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number14