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Cellosaurus SiHa (CVCL_0032)

[Text version]
Cell line name SiHa
Synonyms Siha; SIHA
Accession CVCL_0032
Resource Identification Initiative To cite this cell line use: SiHa (RRID:CVCL_0032)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Virology: Contains a complete HPV16 genome with a disruption of the E2 region and a small deletion (PubMed=24201445).
Doubling time: 2.6 days (PubMed=29156801); 17 hours (Note=In 10% FBS), 21 hours (Note=In 5% FBS) (PubMed=30760827).
Microsatellite instability: Stable (MSS) (Sanger).
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Omics: CNV analysis.
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: Secretome proteome analysis by 2D-DE/MS.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
HLA typing Source: PubMed=26589293
Class I
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0
East Asian, North84.36
East Asian, South15.4
South Asian0
European, North0
European, South0.24
Disease Human papillomavirus-related cervical squamous cell carcinoma (NCIt: C27676)
Squamous cell carcinoma of the cervix uteri (ORDO: Orphanet_213767)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C0TY (SiHa-APOA1)CVCL_X529 (SiHa-NTRpuro)CVCL_K031 (SiHa/cDDP)
CVCL_XB80 (SiHa/VP16)CVCL_WU75 (TL [Human SiHa contaminated cell line])
Sex of cell Female
Age at sampling 55Y
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CCRID; Cosmic-CLP; KCLB; PubMed=25877200; PubMed=29156801

D16S5398,12 (PubMed=29156801)
12 (AddexBio; ATCC; CCRID; Cosmic-CLP; KCLB; PubMed=25877200)
Penta D9,12
Penta E10,12
TPOX8 (AddexBio; ATCC; CCRID; Cosmic-CLP; KCLB; PubMed=25877200)
8,9 (PubMed=29156801)

Run an STR similarity search on this cell line
Web pages https://www.proteinatlas.org/learn/cellines

PubMed=5529598; DOI=10.3181/00379727-135-35091a
Friedl F., Kimura I., Osato T., Ito Y.
Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology.
Proc. Soc. Exp. Biol. Med. 135:543-545(1970)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

Yee C., Krishnan-Hewlett I., Baker C.C., Schlegel R., Howley P.M.
Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
Am. J. Pathol. 119:361-366(1985)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3029430; DOI=10.1128/JVI.61.4.962-971.1987
Baker C.C., Phelps W.C., Lindgren V., Braun M.J., Gonda M.A., Howley P.M.
Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.
J. Virol. 61:962-971(1987)

PubMed=3029760; DOI=10.1073/pnas.84.4.1070
Durst M., Croce C.M., Gissmann L., Schwarz E., Huebner K.
Papillomavirus sequences integrate near cellular oncogenes in some cervical carcinomas.
Proc. Natl. Acad. Sci. U.S.A. 84:1070-1074(1987)

Somers K.D., Merrick M.A., Lopez M.E., Incognito L.S., Schechter G.L., Casey G.
Frequent p53 mutations in head and neck cancer.
Cancer Res. 52:5997-6000(1992)

PubMed=10423141; DOI=10.1099/0022-1317-80-7-1725
Meissner J.D.
Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines.
J. Gen. Virol. 80:1725-1733(1999)

PubMed=15531914; DOI=10.1038/sj.onc.1208235
Baldus S.E., Schwarz E., Lohrey C., Zapatka M., Landsberg S., Hahn S.A., Schmidt D., Dienes H.-P., Schmiegel W.H., Schwarte-Waldhoff I.
Smad4 deficiency in cervical carcinoma cells.
Oncogene 24:810-819(2005)

PubMed=17311676; DOI=10.1186/1471-2164-8-53
Kloth J.N., Oosting J., van Wezel T., Szuhai K., Knijnenburg J., Gorter A., Kenter G.G., Fleuren G.J., Jordanova E.S.
Combined array-comparative genomic hybridization and single-nucleotide polymorphism-loss of heterozygosity analysis reveals complex genetic alterations in cervical cancer.
BMC Genomics 8:53.1-53.13(2007)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20429727; DOI=10.3109/02841861003614343
Sorensen B.S., Toustrup K., Horsman M.R., Overgaard J., Alsner J.
Identifying pH independent hypoxia induced genes in human squamous cell carcinomas in vitro.
Acta Oncol. 49:895-905(2010)

PubMed=21439369; DOI=10.1016/j.mcp.2011.03.003
Schmitt M., Pawlita M.
The HPV transcriptome in HPV16 positive cell lines.
Mol. Cell. Probes 25:108-113(2011)

PubMed=22412903; DOI=10.1371/journal.pone.0032667
Vazquez-Mena O., Medina-Martinez I., Juarez-Torres E., Barron V., Espinosa A., Villegas-Sepulveda N., Gomez-Laguna L., Nieto-Martinez K., Orozco L., Roman-Bassaure E., Munoz Cortez S., Borges Ibanez M., Venegas-Vega C.A., Guardado-Estrada M., Rangel-Lopez A., Kofman S., Berumen J.
Amplified genes may be overexpressed, unchanged, or downregulated in cervical cancer cell lines.
PLoS ONE 7:E32667-E32667(2012)

PubMed=24134916; DOI=10.1186/1755-8166-6-44
McCormack A., Fan J.-L., Duesberg M., Bloomfield M., Fiala C., Duesberg P.H.
Individual karyotypes at the origins of cervical carcinomas.
Mol. Cytogenet. 6:44.1-44.23(2013)

PubMed=23881600; DOI=10.1002/jcp.24433
Ravi M., Sah S., Bhammar R.
Differences of SiHa (human cancer of cervix) and BMG-1 (brain glioma) cell lines as 2D and 3D cultures.
J. Cell. Physiol. 229:127-131(2014)

PubMed=24201445; DOI=10.1101/gr.164806.113
Akagi K., Li J.-F., Broutian T.R., Padilla-Nash H.M., Xiao W.-H., Jiang B., Rocco J.W., Teknos T.N., Kumar B., Wangsa D., He D., Ried T., Symer D.E., Gillison M.L.
Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability.
Genome Res. 24:185-199(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28261610; DOI=10.1155/2017/4180703
Kontostathi G., Zoidakis J., Makridakis M., Lygirou V., Mermelekas G., Papadopoulos T., Vougas K., Vlamis-Gardikas A., Drakakis P., Loutradis D., Vlahou A., Anagnou N.P., Pappa K.I.
Cervical cancer cell line secretome highlights the roles of transforming growth factor-beta-induced protein ig-h3, peroxiredoxin-2, and NRF2 on cervical carcinogenesis.
Biomed Res. Int. 2017:4180703.1-4180703.15(2017)

PubMed=29156801; DOI=10.18632/oncotarget.21174
Kalu N.N., Mazumdar T., Peng S.-H., Shen L., Sambandam V., Rao X.-Y., Xi Y.-X., Li L.-R., Qi Y., Gleber-Netto F.O., Patel A., Wang J., Frederick M.J., Myers J.N., Pickering C.R., Johnson F.M.
Genomic characterization of human papillomavirus-positive and -negative human squamous cell cancer cell lines.
Oncotarget 8:86369-86383(2017)

PubMed=30760827; DOI=10.1038/s41598-018-38315-7
Rosa M.N., Evangelista A.F., Leal L.F., de Oliveira C.M., Silva V.A.O., Munari C.C., Munari F.F., de Macedo Matsushita G., dos Reis R., Andrade C.E., de Padua Souza C., Reis R.M.
Establishment, molecular and biological characterization of HCB-514: a novel human cervical cancer cell line.
Sci. Rep. 9:1913-1913(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35198691; DOI=10.1016/j.dib.2022.107919
Huang Z.-L., Kong W.-J., Wong B.J.-H., Gao H.-H., Guo T.-N., Liu X.-M., Du X.-X., Wong L.-S., Goh W.W.-B.
Proteomic datasets of HeLa and SiHa cell lines acquired by DDA-PASEF and diaPASEF.
Data Brief 41:107919.1-107919.7(2022)

PubMed=35421932; DOI=10.1186/s12885-022-09528-x
He Y., Han S.-B., Liu Y., Zhang J.-J., Wu Y.-M.
Role of APOA1 in the resistance to platinum-based chemotherapy in squamous cervical cancer.
BMC Cancer 22:411.1-411.12(2022)

PubMed=35692560; DOI=10.1080/23723556.2022.2078628
de Lourdes Zuniga-Martinez M., Lopez-Mendoza C.M., Tenorio-Salazar J., Garcia-Carranca A.M., Cerbon-Cervantes M.A., Alcantara-Quintana L.E.
Establishment, authenticity, and characterization of cervical cancer cell lines.
Mol. Cell. Oncol. 9:2078628.1-2078628.10(2022)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cell line collections (Providers) AddexBio; C0008002/372
CLS; 305023
ICLC; HTL98017
KCB; KCB 2013026YJ
KCLB; 30035
Cell line databases/resources CLO; CLO_0009017
MCCL; MCC:0000451
CLDB; cl4298
CLDB; cl4299
CLDB; cl4900
cancercelllines; CVCL_0032
CCRID; 1101HUM-PUMC000294
Cell_Model_Passport; SIDM01093
Cosmic-CLP; 930297
DepMap; ACH-000556
IGRhCellID; SiHa
TOKU-E; 3119
Anatomy/cell type resources BTO; BTO:0002210
Biological sample resources BioSample; SAMN03473215
BioSample; SAMN10989623
CRISP screens repositories BioGRID_ORCS_Cell_line; 1796
Chemistry resources ChEMBL-Cells; CHEMBL3307968
ChEMBL-Targets; CHEMBL612542
GDSC; 930297
PharmacoDB; SiHa_1377_2019
PubChem_Cell_line; CVCL_0032
Encyclopedic resources Wikidata; Q54953470
Experimental variables resources EFO; EFO_0002379
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-3610
GEO; GSM113866
GEO; GSM226740
GEO; GSM226876
GEO; GSM253302
GEO; GSM675738
GEO; GSM685444
GEO; GSM722576
GEO; GSM722577
GEO; GSM723057
GEO; GSM723058
GEO; GSM1670422
GEO; GSM1716411
GEO; GSM1716412
GEO; GSM1716413
GEO; GSM1716414
GEO; GSM1716415
GEO; GSM1716416
GEO; GSM1716423
GEO; GSM1716424
GEO; GSM1716425
GEO; GSM1716426
Polymorphism and mutation databases Cosmic; 760220
Cosmic; 809229
Cosmic; 846184
Cosmic; 877467
Cosmic; 925356
Cosmic; 928911
Cosmic; 930297
Cosmic; 1019306
Cosmic; 1131517
Cosmic; 1193028
Cosmic; 1201773
Cosmic; 1324212
Cosmic; 1995627
Cosmic; 2301542
Cosmic; 2660193
Cosmic; 2791018
Cosmic; 2816217
IARC_TP53; 21696
Progenetix; CVCL_0032
Proteomic databases PRIDE; PXD001806
PRIDE; PXD005466
PRIDE; PXD029773
PRIDE; PXD030304
PRIDE; PXD030885
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number43