Lung Carcinoma Cell Lines

NCI-H2009 [Cellosaurus entry CVCL_1514]

Lung Cancer Cell Line

Description	A hyper-diploid cell line from lung, metastatic site, lymph node adenocarcinoma, held at ATCC. Karyotyped in collaboration with the Sanger Institute.

Notable Features	Extremely rearranged with 27 structural abnormalities. Translocations involve chromosomes 1, 2, 3, 4, 5, 6, 7, 11, 12, 13, 14, 15, 16, 17, 19, 20, 22. No normal chromosomes 1, 6, 13, 14, 18, 22 were found. Loss of chromosome 13 and del (13). Most rearrangements unbalanced, but two reciprocal translocations observed – t(3;6)(q24;q21) and t(12;17)(p10;p10). All cells contained double minutes (DM), ranging from 1-8 per cell. Consistent DM were found for chromosomes 13, 18, 5, 16.

Representative SKY Karyotype


48(47-49), X, -X, i (1p), der(1)t(13;1;X), der(1)t(1;21), der(1)del(1), 2x2, 3x1, der(3)t(3;6); 4x1, der(4;19)(p10;p10?), der(4)t(4;13), 5x1, i(5p)x2, der(6)t(3;6), 7x1, der(7)t(6;7)dup(7)(q21?-qter), 8x1, i(8q), 9x2, 10x2, 11x1, der(11)t(11;13), der(11)t(6;11), 12x1, der(12;17)(p10;p10), der(13)del(13), der(5;14)(q10-qter :: q?), der(14)t(X;14), 15x1, der(15)t(15;2;19), 16x1, der(16)t(2;16), der(16)del(16), 17x1, der(12;17)(q10;q10), i(18q), 19x1, der(19)t(17;19;1), 20x1, der(9;20)(q10;q10?), der(20)t(16;20), 21x1, der(22)t(5;22), der(22)t(13;22), dmin * reciprocal translocation ** reciprocal translocation


Additional Information