Lung Carcinoma Cell Lines

NCI-H1770 [Cellosaurus entry CVCL_1493]

Lung Cancer Cell Line

Description	A near-triploid cell line from lung, metastatic site, lymph node carcinoma, held by the ATCC. Karyotyped in collaboration with the Sanger Institute.

Notable Features	Near-triploid, with a relatively simple karyotype. Structural abnormalities include three nonbalanced translocations, involving chromosomes 2, 4, 12, 17, and i(1p). One of the rearrangements is a big hsr of chromosome 2 material inserted into chromosome 12. FISH analyses using BAC comprising the NMYC gene (2p23) shows a massive amplification of NMYC (Fig. 2). Losses involve chromosomes 1p, 3, 4, 5, 6, 10, 12, Y; gains involve chromosome 7, 21, 22.

Representative SKY Karyotype


65(61-65) XX, -Y, 1x2, i(1p), del(1)(p1-pter)x2, 2x2, der(2)t(2;4), 3x2, 4x1, 5x2, 6x2, 7x4, 8x2, 9x3, 10x2, 11x3, 12x1, der(12)dup(12)(q14-qter)t(12;2;12)hsr(2), 13x3, 14x2, 15x3, der(15)del(15)(q23?-qter), 16x3, 17x2, der(12;17)(p10;q10), 18x3, 19x3, 20x3, 21x4, 22x2, dmin

	Amplification of NMYC, see above

Additional Information