| Abstract |
A growing body of evidence indicates that microglia, resident innate
immune cells in the brain, play a role in host defense and tissue repair,
and function as active contributors to neuron damage in neurodegenerative
disease. BV-2 microglial cells immortalized by a v-raf/v-myc recombinant
retrovirus (J2) have been widely used as a microglial cell model, but
there are no reports about the chromosomal characteristics of this cell
line such as a gain or loss in DNA copy number. In this report we
conducted a genome-wide determination of chromosomal aberrations in BV-2
microglial cells using a high-throughput, oligonucleotide array-based
comparative genomic hybridization (oaCGH) technique. A segmentation method
was used to divide each chromosome into segments whose probe sequences
share the same relative DNA copy number on average. The genomic location
of each segment was determined using the mouse genome database (UCSC mm8,
NCBI Build 36). Chromosome 4 was found to have the largest gain which
located in the region of chr4 : 3377972-111570775, and chromosome 3 had
the largest loss segment missing from the region of chr3 : 3445973-
86952997. Segments possessing more DNA copies than normal by one copy
(average of log ratios in segment >0.585) were observed in chromosomes 4
and 19 while segments having less DNA copies by one copy (average of log2
ratios <-1) were detected in chromosomes 1, 2, 11 and 13.
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