| Publication number |
CLPUB00764 |
|---|
| Authors |
Axelos M., Curie C., Mazzolini L., Bardet C., Lescure B. |
|---|
| Title |
A protocol for transient gene expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures. |
|---|
| Citation |
Plant Physiol. Biochem. 30:123-128(1992) |
|---|
| Abstract |
Isolation and maintenance of an Arabidopsis thaliana cell suspension
culture, protoplast preparation from these cells and plasmid DNA
transfection into the protoplasts by the polyethyleneglycol procedure are
described. In the reported examples, the DNA contained chimaeric genes
consisting of the coding region of the beta-glucuronidase (GUS) reporter
gene, fused to the cauliflower mosaic virus 35S promoter or to the
promoter from the Arabidopsis elongation factor EF-1 alpha gene. Following
DNA uptake into protoplasts, maximal GUS activity was observed after 50 to
60 h. The measured GUS activity indicated high transient expression of the
tested chimaeric genes.
|
|---|
| Cell lines |
CVCL_D5GW; T87 [A.thaliana] |
|---|
