| Abstract |
One hybridoma clone, 2C9 which produces a monoclonal antibody (IgG1)
reacting with prostate cells was established from a fusion using mouse
splenic cells immunized with LNCaP cells, isolated from a metastatic
lesion of a human prostatic carcinoma (PCa). The 2C9 antibody specifically
reacted with LNCaP cells, and not with other cell lines including another
prostatic carcinoma cells, non-malignant prostatic cells, and normal renal
cells by mixed heamadsorption assay. On immuno-histochemical staining of
various human tissues, 2C9 antibody stained normal and hypertrophic
prostate tissues, but not any of normal tissues. Additionaly, 2C9 antibody
stained poorly differentiated prostate tumors which were not stained with
anti prostate specific antigen (PSA) and anti prostate-acid phosphatase
(PAP) antibodies. The staining pattern with 2C9 antibody differed from
those with anti PSA or anti PAP antibodies. The 2C9 antibody immuno-
precipitated an antigen with a molecular weight of approximately 92 kDa in
the extract from LNCaP cells labeled with 35S-methionine. On sandwich
ELISA using 2C9 antibody, 2C9 antigen was detected in the LNCaP cell
lysate. These results suggest that 2C9 antibody recognizes a different
antigen from PSA and PAP in prostate tissue, and may be useful for the
diagnosis of PCa not detected with anti PSA and anti PAP antibody.
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