| Publication number |
CLPUB00750 |
|---|
| Authors |
George G.A. |
|---|
| Title |
Establishment and characterisation of cell lines from the caerulean damsel, Pomacentrus caeruleus. |
|---|
| Citation |
Thesis PhD (2017); Cochin University of Science and Technology; Kochi; India |
|---|
| Web pages |
http://eprints.cmfri.org.in/id/eprint/13915 |
|---|
| Abstract |
Cell lines and primary cell cultures from fish tissues have been used for
research in disease diagnosis and cytotoxicity evaluation of environmental
pollutants. The principal aim of this work was the development of
continuous cell lines from the caerulean damsel, Pomacentus caeruleus. To
achieve this, tissues were first sourced from donor fishes, devoid of
contaminants using standardised disinfection protocols. Primary cultures
were then initiated and suitable media, additives, dissociation reagents
as well as optimum incubation temperature were determined. Successful
primary cultures were subcultured and passaged to derive continuous cell
lines which were cryopreserved for long term storage. The continuous cell
lines established were characterised by immunotyping using cell type
markers and authenticated to confirm the species of origin using
previously established barcoding techniques. Preliminary applications such
as gene transfection studies and cytotoxicity assays using bacterial
extracellular products were done in addition to ensuring that the cultures
and cryostocks were contamination-free.
|
|---|
| Cell lines |
CVCL_1G93; PC1CpTr CVCL_1G94; PC1F1Ex CVCL_1G95; PC1L1Tr |
|---|
