| Abstract |
To investigate the cellular biological charateristics of bile duct
carcinoma, the first cell line of extrahepatic bile duct carcinoma, named
as QBC939, was established in China. The cell line was histologically
similar to the originai tumor. Electron microscopic studies indicated the
presence of characteristic junctions between cells, microvilli on the cell
surface, microfilaments and microtube in cytoplasm. Immunohistochemistry
of cultured cells and reconstituted tumor in nude mouse showed that the
cell line was strongly positive for karatin and desmosome, supporting the
epilhelial origin of the cell line. The study of growth kinetics
demonstrated that the QBC939 cell line grew fast with independent and
higher viability. The cytogenetic study revealed that the abnormality in
number and structure, such as fracture, transplantation, loss and insert
of chromosomes, presented in the cell line. Soft sepharose culture and
nude mouse xenografts indicated that this cell line was strungly
tumorigenic.
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