Cellosaurus logo
expasy logo

Cellosaurus publication CLPUB00725

Publication number CLPUB00725
Authors Baglia L.A.
Title Survey of the secretion of immunoreactive pituitary proteins by transformed serum-free B lymphoblastoid cell lines.
Citation Thesis PhD (1993), Ohio State University, United States
Web pages https://rave.ohiolink.edu/etdc/view?acc_num=osu1487842372898166
Abstract The purpose of this study was to determine if the serum-free Burkitt lymphoma cell line, sfRamos and other serum-free B lymphoblastoid cell lines established in this study, produces hormone-like proteins that may be autocrine growth factors. To accomplish this, five Burkitt lymphoma cell lines, one B cell lymphoma cell line, and one LCL were adopted to grow continuously in serum-free medium. These serum-free cell lines produce autocrine growth factors. Spent medium from the serum-free EBV-negative Burkitt lymphoma cell line, sfRamos, has lactogenic hormone bioactivity and antiserum IgG from both rabbit anti-PRL and anti-GH inhibits the proliferation of the sfRamos cells. This study demonstrates the presence of immunoreactive hormone polypeptides in sfRamos, sfJiyoye, sfAWRamos, sfRAJI, sfBT spent medium and sfRamos cell lysates, but not in fresh serum-free medium.
Cell lines CVCL_C5DM; sfAWRamos
CVCL_C5DK; sfJiyoye
CVCL_C5DL; sfRaji
CVCL_WS53; sfRamos