| Abstract |
The purpose of this study was to determine if the serum-free Burkitt
lymphoma cell line, sfRamos and other serum-free B lymphoblastoid cell lines
established in this study, produces hormone-like proteins that may be autocrine
growth factors. To accomplish this, five Burkitt lymphoma cell lines, one B cell
lymphoma cell line, and one LCL were adopted to grow continuously in serum-free
medium. These serum-free cell lines produce autocrine growth factors.
Spent medium from the serum-free EBV-negative Burkitt lymphoma cell
line, sfRamos, has lactogenic hormone bioactivity and antiserum IgG from both
rabbit anti-PRL and anti-GH inhibits the proliferation of the sfRamos cells. This
study demonstrates the presence of immunoreactive hormone polypeptides in
sfRamos, sfJiyoye, sfAWRamos, sfRAJI, sfBT spent medium and sfRamos cell
lysates, but not in fresh serum-free medium.
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