| Publication number |
CLPUB00723 |
|---|
| Authors |
Doymaz M.Z., Bulut H., Ozdarendel A., Bolat Y. |
|---|
| Title |
Production and characterization of monoclonal anti-ovalbumin antibodies. |
|---|
| Citation |
Turk. J. Med. Sci. 30:411-416(2000) |
|---|
| Web pages |
https://journals.tubitak.gov.tr/medical/vol30/iss5/2/ |
|---|
| Abstract |
In this study, the results of anti-ovalbumin (OVA) monoclonal antibody
production and characterization are reported. Splenocytes from OVA-
hyperimmune Balb/C mouse were fused with F0 myeloma cells and resulting
hybridoma cells were selected in hypoxanthine-aminopterine-thymidine
containing medium. A total of 37 HAT resistant hybridomas were obtained.
Upon testing the hybridoma supernatants in OVA and mouse immunoglobulin G
specific enzyme-linked immunosorbent assays, we observed that two of the
hybridomas secreted OVA specific antibodies. The hybridomas produced IgG
class antibodies. The hybridomas were grown and the supernatants from the
cultures were also tested in a Western Blot assay. The results of ELISA
were confirmed with Western Blot assays. The results indicate that the
mAbs produced in this study reacted with the continuous (non-
conformational) epitopes present on OVA.
|
|---|
| Cell lines |
CVCL_C3LN; BG11 |
|---|
