| Abstract |
Objective: To establish an etoposide-resistant human small cell lung
cancer cell line H446/VP and study its biological characteristics.
Methods: H446/VP cell line was obtained by exposing at intervals and
repeatedly to high dose of etoposide for 7 months. The morphological
feature, ultrastructure and growth characters were examined. Flow cytometry
was used to evaluate the cell cycle distribution of H446/VP and its
parental cell line. MTT assay was used to measure the cross-resistant
profile of H446/VP cells.
Results: Under light microscope, H446/VP cells was similar with H446 cells
stained by hematoxylin and eosin. While observed by transmission electron
microscope, there were more malformed nuclei, abundant mitochondria,
ribosomes and endoplasmic reticular in H446/VP cells. H446/VP cells grew
more slowly than H446 cells and their population doubling time was 35.7
hours which was 7.9 hours longer than H446 cell line. Cell cycle
distribution of H446/VP cells has changed compared with parental cells.
The percentage of cells in Go/G, phase increased to 65.12 +- 1.67% , while
the percentage of cells in G2/M phase decreased to 3.99 +- 1.49%. The
resistance index of H446/VP cells to etoposide was 17.8 and the cells
showed various cross-resistance to ADR, NVT, VCR, MMC and CDDP, the
resistance indexes were 8.9, 5.4, 4.7, 4.4 and 1.7, respectively. The
cells remain sensitive to 5-Fu, MTX, CTX, and so on.
Conclusion: A multidrug resistance cell line H446/VP from human small cell
lung cancer is established. It can be used for further experiments.
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