Publication number |
CLPUB00706 |
Authors |
Noga E.J. |
Title |
Studies on channel catfish virus passaged in cell cultures of the walking catfish. |
Citation |
Thesis MSc (1977); Florida Atlantic University; Boca Raton; USA |
Web pages |
http://purl.flvc.org/fcla/dt/13896 |
Abstract |
Gonadal (GD1I), kidney (K1K) and gill (G1B) cell lines were established
from tissues of the walking catfish using an improved procedure. These
cell lines were found to be susceptible to channel catfish virus (CCV)
infection. A virus sample (HPV) propagated for 59 passages at low dilution
in K1K cells was found to be of lower virulence for young channel catfish
than two other field strains of CCV (LPV) with fewer in vitro passages.
Injection of channel catfish with HPV protected these fish from subsequent
challenge with the more virulent LPV. Challenge of walking catfish with
several different CCV samples including wild strains and Clarias-
propagated virus failed to produce any mortalities.
|
Cell lines |
CVCL_5506; G1B CVCL_S929; GD1I CVCL_S935; K1K |