| Publication number |
CLPUB00705 |
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| Authors |
Poyer J.C. |
|---|
| Title |
The establishment and study of two elasmobranch cell lines. |
|---|
| Citation |
Thesis MSc (1992); Florida Atlantic University; Boca Raton; USA |
|---|
| Web pages |
http://purl.flvc.org/fcla/dt/14795 |
|---|
| Abstract |
The present study describes the first cell lines produced from members of
class Chondrichthyes. Explants of brain tissue from Carcharhinus
falciformis (silky shark) and Ginglymostoma cirratum (nurse shark) were
incubated in a mammalian medium modified with the addition of urea,
trimethylamine N-oxide, NaCl, and bovine serum. Primary monolayers were
passaged with 0.025% trypsin in a modified saline solution. Silky shark
cells grew optimally at 29C. The population doubling time for C.
falciformis cells at passage 29 was 67 hours. For G. cirratum cells at
passage 6 the population doubling was 84 hours. Silky shark cells grew
over a broad range of osmolalities from 315 mOsm to a 1664 mOsm with
optimal growth at 650 mOsm. A medium containing 10% dimethylsulfoxide
allowed for cryopreservation with greater than 65% viability upon recovery.
Current theories of elasmobranch osmoregulation are discussed in light of
experimental data collected from studies conducted on the silky shark cell
line.
|
|---|
| Cell lines |
CVCL_B7QE; NSB CVCL_B7QD; SSB |
|---|
