| Abstract |
Three new cell lines were established from explants of fin (GCF), snout
(GCS-2), and swim bladder (GCSB) obtained from two grass carp fry,
Ctenopharynogodon idellus. The GCSB cells were fibroblastic and the GCF
and GCS-2 cells exhibited mixed morphology. The new cell lines have been
successfully subcultured fifty times. The three cell lines were maintained
at temperatures between 20-36 Celsius with the optimum 28-32 Celsius. The
plating efficiencies for GCF, GCS-2, and GCSB were determined to be
approximately 13, 7, and 4%, respectively. The three cell lines exhibited
similar chromosome distributions which ranged from 39 to 51 with a modal
number of 48. The cell lines were free of mycoplasmal, bacterial, and
fungal contamination at the tenth and thirty-fifth passages. These cells
were tested for their susceptibility to eight fish viruses. The chum
salmon virus (CSV), golden shiner virus (GSV), infectious hematopoietic
necrosis virus (IHNV), infectious pancreatic necrosis virus (IPNV), and
spring viremia of carp virus (SVCV) were found to replicate in all three
cell lines. However, channel catfish virus (CCV), channel catfish reovirus
(CRV), and chinook salmon paramyxovirus (CSP) failed to replicate in these
cells.
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