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Cellosaurus publication CLPUB00701

Publication number CLPUB00701
Authors Gioanni J., Mazeau C., Zanghellini E., Milano G., Fischel J.-L., Turc-Carel C., Schneider M.
Title Establishment of two human cell lines from the same patient suffering from mammary adenocarcinoma and differentially expressing the MDR gene.
Citation Bull. Cancer 80:472-472(1993)
Abstract We describe a representative cellular model of MDR resistance developed in a patient with breast adenocarcinoma. This model consists of two cell lines CAL 85-1 and CAL 85-2. CAL 85-1 comes from the primary tumor after first chemotherapy (adriamycin, 5-FU, cyclophosphamide). CAL 85-2 is obtained 9 months later, following adjuvant chemotherapy (5-FU, epirubicin, cyclophosphamide) and heavy chemotherapy (melphalan) before auto-grafting of bone marrow, from a chest recurrence. We give the morphological and cytogenetic characteristics of these two lines. For this model, MDR expression was measured by cytofluorimetry at using monoclonal antibodies C 219, JSB 1 and MRK 16. Only the latter shows a difference in MDR expression in favor of CAL 85-2. The efficacy of 3 MDR reversants, verapamil, cyclosporine and S 9788 is consistent with a moderate difference in MDR expression between CAL 85-1 and CAL 85-2. This cellular system seems closer to reality clinical than those previously established in vitro by artificial selective pressure.
Cell lines CVCL_1114; CAL-85-1