| Publication number |
CLPUB00701 |
|---|
| Authors |
Gioanni J., Mazeau C., Zanghellini E., Milano G., Fischel J.-L., Turc-Carel C., Schneider M. |
|---|
| Title |
Establishment of two human cell lines from the same patient suffering from mammary adenocarcinoma and differentially expressing the MDR gene. |
|---|
| Citation |
Bull. Cancer 80:472-472(1993) |
|---|
| Abstract |
We describe a representative cellular model of MDR resistance developed in
a patient with breast adenocarcinoma. This model consists of two cell
lines CAL 85-1 and CAL 85-2. CAL 85-1 comes from the primary tumor after
first chemotherapy (adriamycin, 5-FU, cyclophosphamide). CAL 85-2 is
obtained 9 months later, following adjuvant chemotherapy (5-FU,
epirubicin, cyclophosphamide) and heavy chemotherapy (melphalan) before
auto-grafting of bone marrow, from a chest recurrence. We give the
morphological and cytogenetic characteristics of these two lines. For this
model, MDR expression was measured by cytofluorimetry at using monoclonal
antibodies C 219, JSB 1 and MRK 16. Only the latter shows a difference in
MDR expression in favor of CAL 85-2. The efficacy of 3 MDR reversants,
verapamil, cyclosporine and S 9788 is consistent with a moderate
difference in MDR expression between CAL 85-1 and CAL 85-2. This cellular
system seems closer to reality clinical than those previously established
in vitro by artificial selective pressure.
|
|---|
| Cell lines |
CVCL_1114; CAL-85-1 CVCL_B7MJ; CAL-85-2 |
|---|
