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Cellosaurus publication CLPUB00698

Publication number CLPUB00698
Authors van Bokhoven A.
Title Models for prostate cancer. Molecular characterization and critical appraisal of human prostate carcinoma cell lines.
Citation Thesis PhD (2004), Katholieke Universiteit Nijmegen, Netherlands
Web pages https://repository.ubn.ru.nl/handle/2066/58826
Abstract The aim of this thesis is to define the collection of prostate carcinoma cell lines This is accomplished by verifying the origin of described cell line models as well as a thorough characterization of the models by karyotypic, mutation, and expression analyses. Chapter II. Seven prostate carcinoma cell lines are analyzed for cadherin and catenin expression. Expression of mesenchymal cadherins is observed in cell lines with an impaired E-cadherin adhesion. Possible implications of epithelial/mesenchymal transition for prostate carcinogenesis are discussed. Chapter III. The genetic background of 14 widely used prostate carcinoma cell lines is analyzed by cytogenetics, DNA profiling and mutation analysis. Several supposedly unique cell lines appear to have a common origin. Guidelines for cell line use are discussed. Chapter IV. Two cell lines previously believed to be of prostatic origin are identified as cross-contaminants of T24 bladder carcinoma cells Implications of continued use of cross-contaminants in research are discussed. Chapter V. Comparison of several commercial available profiling kits and the use of publicly available data as reference source for cell line identification are described. The PEAZ-1 cell line believed to be of prostatic origin is identified as a HT-1080 fibrosarcoma derivative. Chapter VI. An extensive literature search for putative prostate carcinoma cell lines and subsequent molecular analysis defines a collection of 17 prostate carcinoma cell lines. Comprehensive analysis of p53 and androgen receptor status combined with expression analysis of differentiation markers is used to determine how accurate these cell lines mimic different stages of prostate cancer. Chapter VII. Detailed spectral karyotypic analysis of prostate carcinoma cell lines and a comparison with chromosomal changes observed in prostate cancer. Chapter VIII. Karyotypic analysis of two non-acinar prostate carcinoma cell lines.
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