| Abstract |
The aim of this thesis is to define the collection of prostate carcinoma
cell lines This is accomplished by verifying the origin of described cell
line models as well as a thorough characterization of the models by
karyotypic, mutation, and expression analyses.
Chapter II. Seven prostate carcinoma cell lines are analyzed for cadherin
and catenin expression. Expression of mesenchymal cadherins is observed in
cell lines with an impaired E-cadherin adhesion. Possible implications of
epithelial/mesenchymal transition for prostate carcinogenesis are
discussed.
Chapter III. The genetic background of 14 widely used prostate carcinoma
cell lines is analyzed by cytogenetics, DNA profiling and mutation
analysis. Several supposedly unique cell lines appear to have a common
origin. Guidelines for cell line use are discussed.
Chapter IV. Two cell lines previously believed to be of prostatic origin
are identified as cross-contaminants of T24 bladder carcinoma cells
Implications of continued use of cross-contaminants in research are
discussed.
Chapter V. Comparison of several commercial available profiling kits and
the use of publicly available data as reference source for cell line
identification are described. The PEAZ-1 cell line believed to be of
prostatic origin is identified as a HT-1080 fibrosarcoma derivative.
Chapter VI. An extensive literature search for putative prostate carcinoma
cell lines and subsequent molecular analysis defines a collection of 17
prostate carcinoma cell lines. Comprehensive analysis of p53 and androgen
receptor status combined with expression analysis of differentiation
markers is used to determine how accurate these cell lines mimic different
stages of prostate cancer.
Chapter VII. Detailed spectral karyotypic analysis of prostate carcinoma
cell lines and a comparison with chromosomal changes observed in prostate
cancer.
Chapter VIII. Karyotypic analysis of two non-acinar prostate carcinoma cell
lines.
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