| Publication number |
CLPUB00685 |
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| Authors |
Petrova Y.I., Kameneva O.V., Zhukova A.I., Scudder L., Gnatenko D.V., Bahou W.F., Komisarenko S.V., Skok M.V. |
|---|
| Title |
The role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site. |
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| Citation |
Centr. Eur. J. Immunol. 33:14-18(2008) |
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| Web pages |
https://www.termedia.pl/Experimental-immunology-The-role-of-proteinase-activated-receptor-3-PAR3-in-mouse-hybridoma-studied-with-monoclonal-antibody-generated-against-thrombin-cleavage-site,10,10091,1,1.html |
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| Abstract |
Thrombin produced upon tissue damage provides a link between
inflammation/blood coagulation and immune reactions through proteinase-
activated receptor (PAR) types 1, 3 and 4. In contrast to PAR1 and PAR4,
functions of PAR3 in cells other than platelets are poorly understood. We
generated mAb 8E8 against fragment 31-47, which includes the thrombin
cleavage site of human PAR3. MAb 8E8 bound mouse platelets and delayed
their aggregation induced by thrombin. This antibody also specifically
stained B-lymphocyte-derived hybridoma in flow cytometry. Thrombin dose-
dependently inhibited hybridoma cell proliferation and so did mAb 8E8 and
PAR3 31-47 fragment, but not the PAR4 activating peptide. It is concluded
that mAb 8E8 recognizes PAR3 expressed in hybridoma and mimics the
inhibitory effect of thrombin on hybridoma proliferation mediated by PAR3.
The data obtained also suggest that, unlike in platelets, targeting PAR3
in mouse B lymphocyte-derived cells doesn't involve PAR4.
|
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| Cell lines |
CVCL_B6RX; 8E8 |
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