| Publication number |
CLPUB00684 |
|---|
| Authors |
Sikora J., Lawniczak M., Dworacki G., Zeromski J. |
|---|
| Title |
Production and partial characterisation of mouse monoclonal antibody against human fibronectin. |
|---|
| Citation |
Centr. Eur. J. Immunol. 22:255-261(1997) |
|---|
| Web pages |
https://eurekamag.com/research/009/249/009249585.php |
|---|
| Abstract |
This paper describes the preparation of a mouse monoclonal antibody JS-1
directed against human fibronectin. This is a fusion product of mouse
myeloma X63Ag8.653 and of spleen cells from a BALB/c mice which had been
immunized with fibronectin isolated from human serum. This antigen was
visualized as two bands (MW 200-250 kD) in SDS-PAGE electrophoresis. ELISA
and immunohistology have shown that the produced antibodies react with
structures containing fibronectin and extracellular protein and belonged
to the IgM class of immunoglobulin. Flow cytometric analysis of the
hybridoma cells had tetraploid DNA content and a high percentage of cells
in the S-phase fraction of the cell cycle.
|
|---|
| Cell lines |
CVCL_B6RW; JS-1 |
|---|
