| Abstract |
The susceptibility to grass carp reovirus (GCRV) of CAB-80, BCC, PHG, GCE
GCTF-2 and GCK-84 (these cell lines were derived from crucian carp
blastula embryos, herbivorous bream caudal fins, loach haploid gastrula
embryos, grass carp gastrula embryos, grass carp caudal fins and grass
carp kidney, respectively), as well as four clones isolated from GCK-84,
were analyzed comparatively. Sensitivity of various degrees was confirmed
in the infected cell lines (except PHG) among which GCK-84 was the most
sensitive. It is demonstrated that GCRV, in vitro, has no strict species
specificity. Typical symptom of muscular hemorrhage could be replicated
when fingerlings of grass carp were infected with the virus which had been
propagated in GCK-84. The virus produced by GCK-84, GCTF-2, CAB-80, BCC
and PHG was titred in GCK-84 cells. The titers (TCID50/ml) were 8.24, 7.36,
2.90, 2.15 and 1.33 respectively. Sicells difference was also
observed in the four clones and GCK-84, with the titers ranging from 6.3
to 9.32. The results prescnted above predict the relative potential
significance in the disease-resistant breeding of fish cell engineering.
Serious damages in GCRV-infected GCK-84 cells could be seen with electron
microscopy. The observed virus was in the shape of spherical particles
having a size of 58 nm in diameter and a core with high electronic density
approximately 38 nm in diameter on the average. The distribution of
virions in cells could be divided into three patterns: existing
individually in cytoplasm, congregating in large crystalline aggregates
bound by membranceous structures, gathering together in an orderly or
disorderly arrangement without a surrounding membrane.
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