| Abstract |
A diploid fish cell line, named GCCF-2, from grass carp caudalfin was
established. The line was cultivated in TC-199 medium with 15% fetal
calf serum containing a moderate amount of antibiotics. The morphology of
GCCF-2 cells is a complex of epithelial-like cells, fibroblastic-like
cells and the medial type cells. The cells can increase five times in five
days and the highest mitotic index of GCCF-2 is 30.04% in two dayse after
plating. An interesting discovery was observed that the cells which had
been kept in the incubator at 20 Celsius for two months without any care
could grow normally after subculture. At the 90th passage, the cells which
had a normal number of chromosomes of grass carp (2n=48) accounted for 86.7%,
so the line was determined as a diploid fish cell line. So far, the
line hes been maintained in proliferating for over 32 months. Using the
technique of BrdU-labeling and sister chromatid differential staining, we
estimate the cell cycle time of mitotie division of GCCF-2 is between 21-33
hours. GCCF-2 was observed to be susceptible infection with grass carp
reovirus (GCRV).
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