Abstract |
The cell strain AHZC 88 of anti-hemorrhage of grass carp has been obtained
by repeatedly UV inducement on strain ZC 7901 from the snout tissue cell
sensitive to hemorrhage of grass carp. Identification of its biological
characteristic showed that its chromosome number was 2n=48, the range of
its suitable temperature was between 4-38 Celsius, the optimal growth was
27 Celsius, and mitotic index reached to maximum in 36 hours. The
infective test with the pathogenic virus of hemorrhage proved that the
cell strain AHZC 88 was not infected with the hemorrhage virus. The
observation under electron microscope showed that there was no virosome in
the cell of strain. The analysis of LDH isoenzyme displayed five zones
which were different from the sensitive cell strain.
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