| Publication number |
CLPUB00673 |
|---|
| Authors |
Chen M.-R., Chen H.-X., Yi Y.-L. |
|---|
| Title |
The establishment of a heteroploid line from crucian carp and its biological characteristics. |
|---|
| Citation |
J. Fish. China 9:121-130(1985) |
|---|
| Web pages |
https://www.china-fishery.com/jfcen/article/abstract/19850202 |
|---|
| Abstract |
This paper deals with the establishment of a heteroploid cell line.
designated CAB-80, which originally comes from the culture of blastulae
embryonic cells of crucian carp (Carassius auratus L.). Some biological
characteristics of this cell line are described briefly. The conditions
used in primary cell culture and subculture are TC-199 medium containing a
moderate amount of antibiotics and 20% calf serum and incubated at
temperature 27-28 Celsius, pH 7.2. A heteroploid cell line has been
established through 100 subculture passage over a period of 24 months. The
cell line consists of epithelial like cells in a great measure and a few
fibroblast like cells. The optimum growth conditions of CAB-80 is at
temperature 22-28 Celsius and pH value range at 7.2-8.2. The time for
doubling CAB-80 cell population requires 21-45 hours. After incubation for
24 hours, the mitotic index of CAB-80 is 29.5% The distribution of
chromosome numbers of 60th passage cells are ranged from 68 to 178, so it
is heteroploid cell line. The experiment shows that the CAB-80 cells have
somewhat cytopathogenic effect to reovirus infection. It may be used as a
bioassay indicator for fish virus.
|
|---|
| Cell lines |
CVCL_B5DI; CAB-80 |
|---|
