| Publication number |
CLPUB00660 |
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| Authors |
Zou C.-Z., Romero L., Turner-Gillies E., Shapiro B.A., McWilliams-Koeppen H.P., Chase B. |
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| Title |
Genetically modified renal proximal tubule epithelial cells. |
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| Citation |
(In technical document) ATCC application note AP-082021-v05; pp.1-8; ATCC; Manassas; USA (2021) |
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| Web pages |
https://www.atcc.org/resources/application-notes/genetically-modified-renal-proximal-tubule-epithelial-cells |
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| Abstract |
Solute carrier kidney transporter (SLC) protein models are essential for
determining the renal clearance of drugs, environmental compounds, and
endogenous molecules. However, to date there is a lack of preclinical cell-
based models; many current models do not express critical SLC proteins and
those that do are not of mature adult kidney tissue origin. We created
three different hTERT-immortalized human primary renal proximal tubule
epithelial cells (RPTEC) that stably express the OAT1, OCT2, and OAT3
proteins. We then tested the ability of these cells to intracellularly
convey known SLC protein substrates.
|
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| Cell lines |
CVCL_RM67; RPTEC/TERT1 OAT1 CVCL_VR68; RPTEC/TERT1 OAT3 CVCL_RM66; RPTEC/TERT1 OCT2 |
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