| Publication number |
CLPUB00649 |
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| Authors |
Goodman C.L., Corcoran J., Saathoff S.G., Ringbauer J.A. Jr., Guo Y., Stamps M., Bonning B.C., Stanley D.W. |
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| Title |
Establishment and transcriptomic characterization of cell lines and sublines from the small hive beetle, Aethina tumida (Coleoptera: Nitidulidae). |
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| Citation |
In Vitro Cell. Dev. Biol. Anim. 57 Suppl. 1:S41-S42(2021) |
|---|
| Web pages |
https://www.ars.usda.gov/research/publications/publication/?seqNo115=382798https://link.springer.com/article/10.1007%2Fs11626-021-00566-6 |
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| Abstract |
The small hive beetle (SHB), Aethina tumida, originated in sub-Saharan
Africa and now has a world-wide distribution. Adult SHBs deposit their
eggs in honey bee colonies and the larvae feed on honey, pollen and honey
bee larvae. Over time this negatively impacts honey bee populations, which
translates into a major economic impact on agriculture. A number of
measures have been implemented to control SHBs, including the use of
biological agents. Current efforts focus on developing novel, SHB-specific
insecticides, which could be enhanced with tools to screen candidate
compounds against cell lines derived from SHB tissues. Here we report on
two continuously replicating SHB cell lines: BCIRL-AtuE-1127-SGS from eggs
and BCIRL-AtuNE-1129-SGS from eggs and neonate larvae. Each line consists
of a variety of cell morphotypes, including firmly attached asymmetrical
cells and loosely-attached spherical cells. Numerous sublines were
produced by limiting dilution to isolate different cell morphotypes; 20
sublines were selected and cryopreserved. Of these, 3 were selected from
the AtuNE-1129 parental line and 6 from the AtuE-1127 line for further
molecular and genetic characterization. DNA barcoding confirmed that the
sublines were derived from SHB tissues. Growth curves indicated their
doubling times ranged from 29.2 h to 68.4 h. Gene expression profiles were
evaluated in three sublines via RNA-Seq. The transcriptomic libraries and
gene expression profiles will aid in the identification of potential
insecticidal targets present in the cell lines. Here we present a small
subset of genes expressed in these cell lines that could support
insecticide development programs, including: various members of ABC
transporter families A-G; calcium transporters, such as plasma membrane
and endoplasmic reticulum calcium ATPases; and GPCRs with
neurophysiological functions, such as octopamine and pyrokinin receptors.
|
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| Cell lines |
CVCL_B0WH; BCIRL-AtuE-1127-SGS CVCL_B0WI; BCIRL-AtuNE-1129-SGS |
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