| Publication number |
CLPUB00648 |
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| Authors |
Rock D.M., Horne W.A., Stoehr S.J., Hashimoto C., Zhou M., Cong R., Palma A., Hidayetoglu D., Offord J.D. |
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| Title |
Does alpha1E code for T-type calcium channels? A comparison of recombinant alpha1E calcium channels with GH3 pituitary T-type and recombinant alpha1B calcium channels. |
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| Citation |
(In book chapter) Low-voltage-activated T-type calcium channels; Tsien R.W., Clozel J.-P., Nargeot J. (eds.); pp.279-289; Adis International; Chester; United Kingdom (1998) |
|---|
| Abstract |
Expression of alpha1E (E class) subunits in Xenopus oocytes or in
mammalian cell lines produces calcium channels that show rapid
inactivation. It was originally proposed that alpha1E was the alpha
subunit for low-voltage-activated (LVA) calcium channels. Under identical
recording conditions, we compared biophysical and pharmacological
properties of alpha1E expressed in HEK293 cells with alpha1B (B class)
expressed in the same cell line and LVA calcium channel currents in a rat
pituitary cell line (GH3). Alpha1E calcium channels showed biophysical
properties that were similar to those of alpha1B channels, activation
voltages that were depolarised relative to GH3 T-type current and potent
block by Cd2+ and the non-selective calcium channel toxin omega-Aga-IIIA.
These features of alpha1E calcium channels are similar to those of R-type
calcium channels described in cerebellar granule neurons, and not to GH3
T-type or other LVA calcium channels.
|
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| Cell lines |
CVCL_B0KZ; 192C CVCL_B0KY; 34893 2L |
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