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Cellosaurus publication CLPUB00643
| Publication number | CLPUB00643 |
|---|---|
| Authors | Shi C.-H., Zhang H., Xi L., Wang X.-W., Wang L.-M., Zhao Y. |
| Title | Establishment of a stable cell line expressing the fusion protein of Mycobacterium tuberculosis Hsp65 and IL-2. |
| Citation | Zhongguo Ren Shou Gong Huan Bing Xue Bao 24:718-721(2008) |
| Web pages | http://www.rsghb.cn/EN/Y2008/V24/I08/718 |
| Abstract | The genes encoding Mycobacterium tuberculosis Hsp65 and IL-2 were cloned into eukaryotic expression vector pcDNA3.1 and transformed to E.coli DH5a. Then, the positive recombinant plasmid Hsp65-IL-2-pcDNA 3.1 was selected and transfected to P815 cells (H-2 d) whose genetic background was identical to that of BALB/c mice. In this way, one positive cell clone was selected by G418, and the specific mRNA of the fusion protein was detected by RT-PCR. By immunofluorescence technique (IFT) the P815 cells transfected with recombinant plasmid showed strong fluorescence, indicating the expression of fusion protein in this cell. It is apparent that a stable cell line expressing M.tuberculosis Hsp65 and IL-2 proteins in P915 cells is obtained, which may be used as the optimal target cell for CTL experiment. |
| Cell lines | CVCL_A9JC; P815 Hsp65-IL-2 |