| Abstract |
To improve the titers of porcine circovirus 2 (PCV2), PK15 cells were
cloned using the limiting dilution method and the susceptible cells to
PCV2 were selected by indirect immunofluorescence assay. In result, one
clone, PK15-B1, could reliably produce PCV2 with high titers was selected
from heterogeneous PK15 parental cells. The viral titer could be as high
as 107.0TCID50/mL after the PK15-B1 cells inoculated with 100 TCID50 for
3 days. The levels of the viral titer and growth speed in PK15-B1 cells
were significantly higher than those in PK15 parental cells. Moreover,the
clone cells did not contaminated with porcine circovirus 1, bovine viral
diarrhea virus, classical swine fever virus, encephalomyocarditis virus or
mycoplasma. The results indicated that the PK15-B1 cell clone is reliably
permissive to PCV2 replication and could be used to study the biological
characterization of PCV2 and replication of PCV2 in vitro.
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