| Abstract |
In 1958-59 we reported on our first attempt to develop a usable system of
markers for quantitative genetic studies employing human cell cultures.
Two sequential mutant lines were isolated, the first D98/AG, with
approximately 100-fold increased resistance to 8-azaguanine (AG) but
retaining sensitivity to 8-azaguanosine (AGR), and the second D98/AGR,
with 60-fold higher resistance to AGR. The mutation rates from the
parental line D98S to D98/AG and from D98/AG to D98/AGR were determined,
and the effect of ultraviolet light (UV) on one of these mutational events
was examined. Since that time this mutational system has been further
extended and refined, the enzymatic basis of the mutant phenotypes
elucidated, and the system evaluated for a variety of genetic
applications, including mutagenicity and DNA mediated transformation
studies.
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