| Publication number |
CLPUB00636 |
|---|
| Authors |
Becker J.E., de Nechaud B., Potter V.R. |
|---|
| Title |
Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesis. |
|---|
| Citation |
(In book chapter) Onco-developmental gene expression; Fishman W.H., Sell S. (eds.); pp.259-270; Academic Press; New York; USA (1976) |
|---|
| Abstract |
Two epithelial cell lines (McA-RH8994 and McA-RH7777) derived from Morris
hepatomas 8994 and 7777 have been established in culture. Both lines,
like the parent tumors, produce alpha-fetoprotein (AFP). The peak of AFP
secretion into culture medium occurs during the logarithmic phase of the
growth curve. Both lines are capable of growth in serum-free medium
although at a reduced rate when compared to serum-containing medium. In
serum-free medium, addition of dexamethasone (DEX) reduces cell
multiplication and increases AFP production in McA-RH8994 whereas DEX
accelerates cell multiplication and decreases AFP production in McA-RH7777.
McA-RH7777 produces AFP and albumin (Alb) at 600:1. McA-R118994 begins to
secrete alpha-M-fetoprotein (AMFP) either after addition of
glucocorticoids or at the end of the log phase of growth. The availability
of these cell lines, both producing "transitory cell antigens" as defined
by Uriel but having different responses to DEX, provides additional tools
for the study of the "unbalanced blocked ontogeny" concept proposed by
Potter.
|
|---|
| Cell lines |
CVCL_0444; McA-RH7777 CVCL_3396; McA-RH8994 |
|---|
