| Publication number |
CLPUB00624 |
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| Authors |
Ackermans F., Hermand P., Vincenzotto C., Mouton C., Bazin H. |
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| Title |
Monoclonal antibodies against Bacteroides gingivalis ATCC 33277. |
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| Citation |
J. Dent. Res. 68:626.A138-626.A138(1989) |
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| Web pages |
https://journals.sagepub.com/doi/abs/10.1177/00220345890680040501 |
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| Abstract |
B.gingivalis appears to be positively associated with several of the
periodontal diseases. This study was undertaken to obtain monoclonal
antibodies (Mabs) against B.gingivalis to characterize immunologically
important constituents and to establish comparison with other periodontal
bacteria. After intrafootpad immunization with B.gingivalis ATCC 33277,
popliteal lymph nodes from a LOU/C rat were collected and fused with non
secreting myeloma cells from LOU/C rats (IR983F). Ten cell lines producing
MAbs reacted with the whole bacteria by ELISA and were called LO-BG-1 to
10. IgG2a and IgG2b were the class and subclass of the MAbs. After
purification from ascitic fluid, purity of the MAbs was tested by
isofocalisation analysis. Purified MAbs were tested by ELISA against
B.gingivalis W83, B.intermedius 5W2, B.melaninogenicus ATCC 15930,
A.actinomycetemcomitans ATCC 29522. A lot of these MAbs reacted strongly
with B.gingivalis and weakly with B.intermedius. LO-BG-1 was tested by
western blotting analyses against external components of B.gingivalis (GBE)
and recognized different proteins between 42 and 47 K. These components
seemed to present similar epitopes.
|
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| Cell lines |
CVCL_A6FQ; LO-BG-1 |
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