| Abstract |
Three-month old LOU/C rats were given two injections of 500 mug of rabbit
immunoglobulin mixed with 200 mul of complete Freund's adjuvant by the
intraperitoneal route. After a rest period of 4 months, the boost of 500
pg of rabbit Ig was done 4 d before the fusion by the intravenous route
(fusion made by L. De Clercq). One rat hybridoma secreting monoclonal
antibodies (MAbs) against rabbit IgG was selected for its binding activity
and its secretion. This MAb (LO-RG-1) is an IgG2a, and can be used to
purify rabbit immunoglobulin. After peroxidase labeling, it can be very
useful as second layer in immunochemistry.
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