| Publication number |
CLPUB00608 |
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| Authors |
Mon E.K., Chaiwongsa R., Klangsinsirikul P., Vongchan P. |
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| Title |
A panel four anti-HSPGs monoclonal antibodies benefits in increasing the specificity in detection of colorectal cancer. |
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| Citation |
Chiang Mai Univ. J. Nat. Sci. 20:e2021055.1-e2021055.14(2021) |
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| Web pages |
https://cmuj.cmu.ac.th/uploads/journal_list_index/2021(3).e2021055.pdf |
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| Abstract |
Colorectal cancer (CRC) is the second leading with main cause of death is
liver and lung metastasis. Using of a combination of genetic and
epigenetic markers are addressed but the results have not been approved in
clinical practice. A set of serum biomarkers has been proposed to increase
accuracy in early diagnosis of CRC. In addition, non-invasive as well as
the best prognostic panel of biomarkers and define predictive biomarkers
for treatment of CRC are all aims of future research. HSPGs is an
important biomolecule involving in cancer cell proliferation,
differentiation, and migration. Membrane HSPGs shed into blood circulation
and matrix in particular circumstance can be used as a specific biomarker
for some cancer cells. In order to evaluate the benefit of a panel of anti-
HSPGs monoclonal antibodies in increasing specificity to detect CRC, four
clones of anti-HSPGs were studied for its specific reaction on various
tumor cell lines by indirect immunofluorescent technique and analyzed by
flow cytometer compared to normal white blood cells. A combination of two
or more clones were focused. The results showed that all four clones
presented a variation in reaction to all solid tumor cell lines tested but
negative to normal white blood cells from different ABO blood groups.
Interestingly, amongst those cells tested, HT29, a colorectal cancer cell
lines were significantly reacted with all four monoclonal antibodies.
Taken together, we proposed a panel of four anti-HSPGs monoclonal
antibodies to be applied in various detection platforms to increase the
specificity in screening of CRC.
|
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| Cell lines |
CVCL_A6AF; 1E2-1D11 CVCL_A6AG; 1E2-2B8 CVCL_A6AH; 1E2-2C9 CVCL_A6AE; 1E4-1C2 |
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