| Publication number |
CLPUB00578 |
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| Authors |
Bronson D.L., Andrews P.W., Vessella R.L., Fraley E.E. |
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| Title |
In vitro differentiation of human embryonal carcinoma cells. |
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| Citation |
(In) Cold Spring Harbor conferences on cell proliferation: teratocarcinoma stem cells, Vol. 10; Silver L.M., Martin G.R., Strickland S. (eds); pp.597-605; Cold Spring Harbor Laboratory Press; New York (1983) |
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| Abstract |
Mammalian cellular differentiation is an irreversible, multistaged process
by which the numerous types of highly specialized adult cells are formed
from totipotent cells of the very early embryo. The study of this process
is one aspect of research efforts in many disciplines to detect factors
that influence differentiation or cause abnormal mammalian development.
Neoplasia may be one abnormality resulting from a derangement or
interference in the differentiation sequence. The mouse teratocarcinoma
system is a model for studies of development and of neoplasia (Pierce 1967;
Stevens 1967). The teratocarcinoma stem (embryonal carcinoma [EC]) cells
are primitive, pluripotent cells that are the malignant component of the
tumor but also are capable of differentiating into benign somatic tissues
representing derivatives of all three germ layers (reviewed by Graham 1977;
Solter and Damjanov 1979; Martin 1980). Thus, these EC cells are valuable
for research in embryogenesis and on the relation between neoplasia and
differentiation. Human nonseminomatous testicular germ cell tumors consist
of one or more components of embryonic (EC, teratocarcinoma, teratoma) or
extraembryonic (choriocarcinoma, yolk sac carcinoma) tissues and, in
specimens of teratocarcinoma and teratoma, of various somatic tissues
with characteristics of benign cells (Mostofi 1977; Nochomovitz et al.
1977). Thus, these tumors have many features in common with the mouse
teratocarcinomas. One objective of our research is to establish cell lines
representing different types of testicular germ cell tumors to examine
the relations between these tumor cells and their potential value in
studies of cellular differentiation and neoplasia. This presentation
describes the results of our studies with two human EC cell lines and
their differentiation potential in vitro.
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| Cell lines |
CVCL_A1EA; 1777N Pr CVCL_2277; 1777N Rpmet |
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