| Publication number |
CLPUB00577 |
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| Authors |
McWilliams-Koeppen H.P., Clinton J. |
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| Title |
A simple and rapid alternative to 51chromium or fluorescent dye loading for quantification of natural killer cell activity: ATCC K-562-GFP cells. |
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| Citation |
(In technical document) ATCC application note 31; pp.1-8; ATCC; Manassas; USA (2017) |
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| Web pages |
https://www.atcc.org/-/media/resources/application-notes/a-simple-and-rapid-alternative-to-51chromium.pdf |
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| Abstract |
Herein we describe a flow cytometry-based assay utilizing K-562 cells
stably expressing green fluorescent protein (ATCC CCL-243-GFP) to
quantify the cytotoxic activity of primary human natural killer cells. The
assay was performed using cryopreserved natural killer cells, with a hands-
on time of under two hours. Specific lysis ranged from 2% to 57%,
depending on the effector: target ratio and the donor (n=2). For many
applications this cell line may provide a more reproducible and preferable
alternative to methods requiring pre-loading of cells with fluorescent or
radioactive materials.
|
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| Cell lines |
CVCL_JM00; K-562-GFP |
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