| Abstract |
A new liver cancer cell line (HCAM) has been characterized; it is
considered as a useful tool in liver cancer research. The HCAM cell line
was established from the primary tumor of a white Swiss albino male mouse
with spontaneous diffuse hepatocellular carcinoma. The current study aimed
to physiologically characterize the cells by comparing it to normal liver
cells from embryo and adult mice. The eighth passage of HCAM cell line was
used in this study. Investigation of liver functions showed high values of
liver enzymes (ALT, AST, GGT, LDH, CRP) in HCAM cell line compared with
normal liver cells that were taken from healthy mice; these enzymes rates
were (52.94, 53.06, 63.63, 390.67, 10.50) U/L respectively (P <=0.05).
High values of tumor marker proteins (AFP, CEA, CA19-9) were also found in
HCAM cell line compared with normal hepatocytes; the rates were (24.23,
15.26, 15.54) U/L respectively (P<=0.05). The hepatic cancer cell line
showing high liver enzyme functional activity, and this makes it useful as
a liver cancer model in functional studies.
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