| Abstract |
A primary culture was set up from of Pieris rapae in TC-100 medium. It was
refed 6 months post explantation, and the cells propagated from the tissues
culture were subcultured two month later. Three substrains, designated
Pr-E(A), Pr-E(B) and Pr-E(C) respectively, developed from the primary cell
line. They differ in morphology, behavior and growth of cells. The cells
of Pr-E(A), Pr-E(B) and Pr-E(C) in their second, fifth and tenth generation
in respect, were preserved at 4 Celsius for 5 months. The Pr-E(A) and Pr-E(B)
cells, which adhere to the bottom of the flasks and grow slowly in
culture, recovered after being transfered to incubator at 28 Celsius, But
the Pr-E(C) cells, which suspend in medium and grow quickly died during
the period.
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