| Abstract |
Lepidopterian cell lines is the primary prerequisites for studying
baculoviruses in culture and baculovirus based vector system for the
expression of recombinant proteins. DZNU-Bm-1 cell line was initiated in
MGM-448 medium supplemented with 10% FBS and 3% heat inactivated B. mori
haemolymph (BMS), originated from larval overies of silkworm Bombyx mori
(L). In the present study, The cells were adapted first adapted to MGM-448
with 10% foetal bovine serum and without haemolymph from MGM-448. Bm-1
cells took about 10-12 months to adapt in haemolymph free MGM-448 medium.
In haemolymph free MGM-448 medium, the cell line became freely suspended
and also showed reduced clumping of cells particularly. This cell lines
adapted to MGM-448 with 10% FBS were subsequently adapted to a cheaper
TNM-FH medium supplemented with 10% FBS. The cell line took about 1-2 months
to get adapted to TNM-FH medium.
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