| Abstract |
The Chinese hamster (Cricetulus griseus) is a species of hamster that was
used as a laboratory animal more than 50 years ago. The Chinese hamster
ovary (CHO) cell line was established in 1957 by Puck et al. and
maintained in ex vivo conditions. CHO cells are now a workhorse for
recombinant biopharmaceutical production. Puck et al. continuously
cultivated lung, kidney, spleen and ovary-derived cells for more than 10
months using serum medium. In this study, we established a cell line from
female Chinese hamster tissues: lung, kidney and ovary. Primary cells were
obtained from these tissues and maintained for several months or more. We
were ultimately able to construct three immortal cell lines, CHL-YN
(fibroblast) from lung, CHK-Q (epitheliocyte) from kidney and CHO-MK
(epitheliocyte) from ovary, respectively. Infinite proliferation of these
cell lines is obtained by spontaneous transformation.
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