| Publication number |
CLPUB00547 |
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| Authors |
Fabien N., Cespuglio R., Burlet-Godinot S., Pages M.-P., Paulin C. |
|---|
| Title |
Co-localization of three neuropeptides and NADPH diaphorase in a small cell lung carcinoma cell line. |
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| Citation |
Cancer J. 7:198-201(1994) |
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| Abstract |
Background: The small cell lung cancer (SCLC) is an epithelial tumour with
the morphological and secretory characteristics of neuroendocrine cells
(neurone-specific enolase, N-CAM, neuropeptides). The establishment of a
SCLC cell line provides an in vitro model to analyze the neuroendocrine
secretion of these malignant cells.
Methods: Bombesin, delta sleep inducing factor (DSIP) and arginine
vasopressin (AVP) were studied by immunohistochemistry and by in situ
hybridization. NADPH diaphorase activity was detected using an
histochemical technique.
Results: All the cells contained bombesin, DSIP, AVP and displayed a NADPH
diaphorase activity with reflects a nitric oxide (NO) synthase activity.
Conclusions: These data present the characteristics of a new SCLC cell
line (CPN) and show the complexity of its neuroendocrine secretion; for
example, the presence of DSIP and NO synthase activity which have not yet
been demonstrated in other SCLC cell lines.
|
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| Cell lines |
CVCL_1146; CPC-N |
|---|
