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Cellosaurus publication CLPUB00543

Publication number CLPUB00543
Authors Min Q., Cao S.-N., Gan Y.-P., Song T.-S., Liu F.-X., Ning Z.-F.
Title Bladder cancer chemotherapy resistant cell harbors stem-like characteristics construction and characterization of T24 resistance strain of bladder cancer.
Citation Clin. Oncol. J. 1:1004.09-1004.12(2019)
Web pages http://www.medtextpublications.com/open-access/pbladder-cancer-chemotherapy-resistant-cell-harbors-stem-like-characteristics-construction-and-characterization-of-t24-resistance-strain-of-bladder-cancerp-160.pdf
Abstract Objective: Establish a human drug-resistant bladder cancer cell line (T24/MMC), which are integral to the initiation, high recurrence and chemo- resistance of bladder cancer. BCSCs are heterogeneous and originate from multiple cell types, including urothelial stem cells and differentiated cell types, including basal, intermediate stratum and umbrella cells, then investigate its characteristics. Methods: Human bladder cancer cell line T24 was established using gradually increased concentration of mitomycin (MMC) in culture. Basic characteristics of both the parental cell line T24 and the drug-resistant cell line (T24/MMC) were analyzed and compared, including the ability of proliferation, colony formation, mobility and invasion, sphere forming ability. Results: Compared with T24, the (T24/MMC) cell grows quickly, and the ability of proliferation, colony formation, mobility and invasion, stemness gene, sphere forming ability increased. Conclusions: Gradually increased concentration of MMC can induce drug- resistant bladder cancer cell line, and it was enriched of cancer stem cells.
Cell lines CVCL_0554; T24
CVCL_XX49; T24/MMC