| Abstract |
Objective: Establish a human drug-resistant bladder cancer cell line
(T24/MMC), which are integral to the initiation, high recurrence and chemo-
resistance of bladder cancer. BCSCs are heterogeneous and originate from
multiple cell types, including urothelial stem cells and differentiated
cell types, including basal, intermediate stratum and umbrella cells, then
investigate its characteristics.
Methods: Human bladder cancer cell line T24 was established using
gradually increased concentration of mitomycin (MMC) in culture. Basic
characteristics of both the parental cell line T24 and the drug-resistant
cell line (T24/MMC) were analyzed and compared, including the ability of
proliferation, colony formation, mobility and invasion, sphere forming
ability.
Results: Compared with T24, the (T24/MMC) cell grows quickly, and the
ability of proliferation, colony formation, mobility and invasion,
stemness gene, sphere forming ability increased.
Conclusions: Gradually increased concentration of MMC can induce drug-
resistant bladder cancer cell line, and it was enriched of cancer stem
cells.
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