| Publication number |
CLPUB00537 |
|---|
| Authors |
Asaoka K. |
|---|
| Title |
Characterization of Bombyx and related insect cell lines by SDS-PAGE and two-dimensional gel electrophoresis. |
|---|
| Citation |
(In book chapter) Invertebrate and fish tissue culture; Kuroda Y., Kurstak E., Maramorosch K. (eds.); pp.278-281; Japan Sci. Soc.; Tokyo; Japan (1988) |
|---|
| Web pages |
https://www.springer.com/de/book/9783642736285 |
|---|
| Abstract |
Characterization of the cell lines derived from Bombyx and related insects
was undertaken using SDS-PAGE and O'Farrell's two-dimensional gel
electrophoresis as a means of discriminating between cell lines derived
from different strains and species of insects. The four B. mori cell lines
from embryos gave nearly identical protein patterns. In the B. mandarina
cell line from embryos, the pattern of most proteins was similar to that
of the proteins of these cell lines, but there were a few species-specific
spots. The two M. brassicae cell lines from fat bodies and the A.
eucalypti cell line from ovaries gave different protein patterns from
those of the Bombyx cell lines. These results indicate that the methods
described can be applied at least for the discrimination and
characterization of the cell lines from different species.
|
|---|
| Cell lines |
CVCL_Z109; Ae CVCL_WK41; SES-Bm-1 30 CVCL_Z697; SES-Bm-1 30R CVCL_WK42; SES-Bm-e 21 CVCL_Z108; SES-BoMo-15A CVCL_Z129; SES-MaBr-3 CVCL_Z131; SES-MaBr-5 CVCL_Z327; SPC-BM-36 |
|---|
