| Abstract |
Six NC, four NN and five RD cell lines, derived from embryos of
Nephotettix cincticeps, N. nigropictus and Recilia dorsalis, respectively
(leafhopper vectors of RDV), have been established employing Liu and
Black's medium modified slightly. All cell lines were epithelial cell
monolayers attached to the bottom surface of plastic flasks, and very
susceptible for RDV infection. Infectivity assays of RDV were made on
vector cell monolayers by the fluorescent antibody focus counting
technique. Infective progeny virions in inoculated cell monolayers were
first detected 12 hr after the initial inoculation. From 12 to 20 hr, the
growth rate of the virus in the cell was exponential with a doubling time
of 96 min.
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